CD284 (TLR4)

Mouse BALB/c IgG2a, κ

Human TLR4-transfected Ba/F3 cell line

The HTA125 monoclonal antibody specifically binds to the human Toll-like receptor 4 (TLR4), also known as CD284. Toll-like receptors (TLR) are type I transmembrane proteins characterized by an extracellular domain containing leucine-rich repeats and a cytoplasmic domain similar to IL-1R family. In humans, ten TLR have been identified so far. The TLR family acts as a pattern-recognition receptor that is an essential component of pathogen recognition and innate immunity. TLR4 has been identified as the receptor for Gram-negative bacterial lipopolysaccharide (LPS). TLR4 mRNA expression has been found in spleen, placenta, ovary, intestine and lung. Using HTA125, TLR4 protein expression has been detected in monocytes, B cells and T cells in human peripheral blood. The human TLR4 gene has been mapped to chromosome 9q32.  The immunogen used to generate the HTA125 hybridoma was human TLR4-transfected Ba/F3 cells. The HTA125 antibody reportedly blocks LPS-triggered, TLR4-mediated  stimulation of cells.

HTA125 The HTA125 monoclonal antibody specifically binds to the human Toll-like receptor 4 (TLR4), also known as CD284. Toll-like receptors (TLR) are type I transmembrane proteins characterized by an extracellular domain containing leucine-rich repeats and a cytoplasmic domain similar to IL-1R family. In humans, ten TLR have been identified so far. The TLR family acts as a pattern-recognition receptor that is an essential component of pathogen recognition and innate immunity. TLR4 has been identified as the receptor for Gram-negative bacterial lipopolysaccharide (LPS). TLR4 mRNA expression has been found in spleen, placenta, ovary, intestine and lung. Using HTA125, TLR4 protein expression has been detected in monocytes, B cells and T cells in human peripheral blood. The human TLR4 gene has been mapped to chromosome 9q32.  The immunogen used to generate the HTA125 hybridoma was human TLR4-transfected Ba/F3 cells. The HTA125 antibody reportedly blocks LPS-triggered, TLR4-mediated  stimulation of cells.

Mouse BALB/c IgG2a, κ

克隆 HTA125 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), Blocking (Reported)

Human (QC Testing)

CD284 (TLR4)

TLR4; CD284; Toll-like Receptor 4; ARMD10

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(11) 1. Akashi S, Ogata H, Kirikae F, et al. Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2. Biochem Biophys Res Commun. 2000; 268(1):172-177. (Clone-specific). 2. Akira S. Toll-like receptors and innate immunity. Adv Immunol. 2001; 78:1-56. (Biology). 3. Beutler B. Tlr4: central component of the sole mammalian LPS sensor. Curr Opin Immunol. 2000; 12(1):20-26. (Biology). 4. Chuang T, Ulevitch RJ. Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells. Biochim Biophys Acta. 2001; 1518(1-2):157-161. (Biology). 5. Chuang TH, Ulevitch RJ. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw. 2000; 11(3):372-378. (Biology). 6. Du X, Poltorak A, Wei Y, Beutler B. Three novel mammalian toll-like receptors: gene structure, expression, and evolution. Eur Cytokine Netw. 2000; 11(3):362-371. (Biology). 7. Paik YH, Schwabe RF, Bataller R, Russo MP, Jobin C, Brenner DA.. Toll-like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells.. Hepatology. 2003; 37(5):1043-1055. (Clone-specific: Blocking). 8. Rock FL, Hardiman G, Timans JC, Kastelein RA, Bazan JF. A family of human receptors structurally related to Drosophila Toll. Proc Natl Acad Sci U S A. 1998; 95(2):588-593. (Biology). 9. Shimazu R, Akashi S, Ogata H, et al. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 1999; 189(11):1777-1782. (Immunogen). 10. Takeuchi O, Kawai T, Sanjo H, et al. TLR6: A novel member of an expanding toll-like receptor family. Gene. 1999; 231(1-2):59-65. (Biology). 11. Zarember KA, Godowski PJ. Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines. J Immunol. 2002; 168(2):554-561. (Biology).

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