BD Pharmingen- Biotin Rat Anti-Human CXCR5 -CD185_BD Pharmingen
产品信息
抗原名称
CD185 (CXCR5)
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
免疫原
Human CXCR5 (CD185)
简单描述
The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5.
商品描述
RF8B2
The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
克隆号
克隆 RF8B2 (RUO)
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl/test
反应种属
Human (QC Testing)
目标/特异性
CD185 (CXCR5)
背景
别名
CD185; Chemokine C-X-C motif receptor 5; BLR1; MDR-15
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B. Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J. 1995; 309(3):773-779. (Biology).
2. Dobner T, Wolf I, Emrich T, Lipp M. Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma. Eur J Immunol. 1992; 22(11):2795-2799. (Biology).
3. Forster R, Emrich T, Kremmer E, Lipp M. Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells. Blood. 1994; 84(3):830-840. (Immunogen).
4. Gunn MD, Ngo VN, Ansel KM, Ekland EH, Cyster JG, Williams LT. A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature. 1998; 391(6669):799-803. (Biology).
5. Kouba M, Vanetti M, Wang X, Schafer M, Hollt V. Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue. FEBS Lett. 1993; 321(2-3):173-178. (Biology).
6. Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B. B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med. 1998; 187(4):655-660. (Biology).
数据库链接
Entrez-Gene ID
643
参考图片
Detection of CXCR5 (CD185) expression on human peripheral lymphocytes by Biotin Rat Anti-Human CXCR5 (CD185). Human peripheral blood mononuclear cells were stained with Biotin Rat Anti-Human CXCR5 (CD185)(Cat. No. 552118) and FITC Mouse Anti-Human CD3 (Cat. No. 555339; Left Panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412; Right Panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Biotin Rat IgG2b, κ Isotype Control (Cat. No. 555847) and FITC-conjugated mouse IgG2a, κ Isotype Control (Cat. No. 555573) or FITC-conjugated mouse IgG1, κ Isotype Control (Cat. No. 555748). The quadrant markers for the bivariate dot plots were set based on the isotype controls.
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