The BD IMag™ Buffer is a 10X solution containing bovine serum albumin, EDTA, and sodium azide in phosphate buffered saline (PBS).  For the quantity needed for the experiment, a working 1X solution is to be prepared by diluting the 10X BD IMag™ Buffer 1:10 with sterile, deionized water for the optimal labeling of leukocytes using BD IMag™ particles and for optimal magnetic cell separation using the BD IMag™ Cell Separation Magnet.  The diluted 1X BD IMag™ buffer should be kept on ice or refrigerated and used within 24 hours.

(RUO)

Cell separation (Routinely Tested)

IMag Buffer 10X

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.