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BD Pharmingen- Purified Rat Anti-Mouse CD326_BD Pharmingen

艾美捷科技 • 2025年08月20日 13:18:24 • 抗体 • 阅读 7 • 标签
产品信息
抗原名称
CD326
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
免疫原
Glycoconjugates from BALB/c mouse-derived TE-71 medullary thymic epithelial cell line
简单描述
The G8.8 monoclonal antibody recognizes CD326/Ep-CAM ( Ep ithelial C ell A dhesion M olecule), also known as gp40 in the mouse and by a variety of names (including GA733-2, CO17-1A, and EGP) in the human.  In the mouse, Ep-CAM is a 40-42 kDa cell-surface type 1 transmembrane glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells and lymph node and splenic dendritic cells. Profiles of Ep-CAM expression on fetal thymocytes and on the CD4[-] CD8[-], CD4[+] CD8[+], CD4[-] CD8[+], and CD4[+] CD8[-] subsets of adult thymocytes have been published.  In unrelated studies, mouse Ep-CAM mRNA was detected in tissues containing epithelial cells (kidney, stomach, intestine, lung, and thymus) and in plasma cells and plasmacytomas, but not in heart, muscle, liver, brain, spleen, B lymphomas, or pre-B lymphomas.  Ep-CAM is a Ca[2+] independent homophilic adhesion molecule that is proposed to play roles in the development and normal function of epithelial tissues and in the progression of carcinomas.
商品描述
G8.8 The G8.8 monoclonal antibody recognizes CD326/Ep-CAM ( Ep ithelial C ell A dhesion M olecule), also known as gp40 in the mouse and by a variety of names (including GA733-2, CO17-1A, and EGP) in the human.  In the mouse, Ep-CAM is a 40-42 kDa cell-surface type 1 transmembrane glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells and lymph node and splenic dendritic cells. Profiles of Ep-CAM expression on fetal thymocytes and on the CD4[-] CD8[-], CD4[+] CD8[+], CD4[-] CD8[+], and CD4[+] CD8[-] subsets of adult thymocytes have been published.  In unrelated studies, mouse Ep-CAM mRNA was detected in tissues containing epithelial cells (kidney, stomach, intestine, lung, and thymus) and in plasma cells and plasmacytomas, but not in heart, muscle, liver, brain, spleen, B lymphomas, or pre-B lymphomas.  Ep-CAM is a Ca[2+] independent homophilic adhesion molecule that is proposed to play roles in the development and normal function of epithelial tissues and in the progression of carcinomas.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
克隆号
克隆 G8.8 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-zinc-fixed (Tested During Development), Electron microscopy, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
CD326 (EpCAM)
背景
别名
Ep-CAM; EGP; EGP-2; Egp314; GA733-2; TROP1; Tacsd1; Tacstd1; Ly74; gp40
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(9) 1. Basak S, Speicher D, Eck S, Wunner W. Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue. J Natl Cancer Inst. 1998; 90(9):691-697. (Biology). 2. Bergsagel PL, Victor-Kobrin C, Timblin CR, Trepel J, Kuehl WM. A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells. J Immunol. 1992; 148(2):590-596. (Biology). 3. Birebent B, Somasundaram R, Purev E. Anti-idiotypic antibody and recombinant antigen vaccines in colorectal cancer patients. Crit Rev Oncol Hematol. 2001; 39((1-2)):107-113. (Biology). 4. Borkowski TA, Nelson AJ, Farr AG, Udey MC. Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells. Eur J Immunol. 1996; 26(1):110-114. (Clone-specific: Immunohistochemistry). 5. Farr A, Nelson A, Truex J, Hosier S. Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. J Histochem Cytochem. 1991; 39(5):345-353. (Immunogen: Electron microscopy, Immunohistochemistry, Immunoprecipitation). 6. Litvinov SV, Balzar M, Winter MJ. Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins. J Biol Chem. 1997; 139(5):1337-1348. (Biology). 7. Nelson AJ, Dunn RJ, Peach R, Aruffo A, Farr AG. The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur J Immunol. 1996; 26(2):401-408. (Clone-specific: Electron microscopy, Immunohistochemistry, Immunoprecipitation). 8. Taguchi N, Hashimoto Y, Naiki M. Abnormal thymic expression of epithelial cell adhesion molecule (EP-CAM) in New Zealand Black (NZB) mice. J Autoimmun. 1999; 13(4):393-400. (Clone-specific: Electron microscopy). 9. Zutter MM. Gastrointestinal carcinoma antigen GA733: target for immunodestruction and potential modifier of invasiveness and chemoresponsiveness. J Natl Cancer Inst. 1998; 90(9):642-644. (Biology).
数据库链接
Entrez-Gene ID
17075

参考图片

Multicolor analysis of the expression of Ep- CAM on splenic T lymphocytes. BALB/c splenocytes were stained with FITC Hamster Anti-Mouse CD3e (Cat. No 553061/553062), PE Rat Anti-Mouse CD25 (Cat. No. 553866), and either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927; Left Panel) or Purified Rat Anti-Mouse CD326 (Cat. No. 552370; Right Panel) in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™; Cat. No. 553141/553142). The G8.8 and isotype control mAb were detected by Biotin Mouse anti-Rat IgG2a (Cat. No. 553894), followed by Streptavidin-APC (Cat. No. 554067). Viable lymphocytes were gated for exclusion of propidium iodide, and the CD25+ subpopulation was selected for analysis of CD3e and Ep-CAM expression. A small population of CD3+CD25+Ep-CAM+ cells were detected (Right Panel), whereas the CD25- T cells do not express Ep-CAM (data not shown). Flow cytometry was performed on a BD FACSCalibur™ (BD Biosciences, San Jose, CA)

Multicolor analysis of the expression of Ep- CAM on splenic T lymphocytes. BALB/c splenocytes were stained with FITC Hamster Anti-Mouse CD3e (Cat. No 553061/553062), PE Rat Anti-Mouse CD25 (Cat. No. 553866), and either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927; Left Panel) or Purified Rat Anti-Mouse CD326 (Cat. No. 552370; Right Panel) in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™; Cat. No. 553141/553142). The G8.8 and isotype control mAb were detected by Biotin Mouse anti-Rat IgG2a (Cat. No. 553894), followed by Streptavidin-APC (Cat. No. 554067). Viable lymphocytes were gated for exclusion of propidium iodide, and the CD25+ subpopulation was selected for analysis of CD3e and Ep-CAM expression. A small population of CD3+CD25+Ep-CAM+ cells were detected (Right Panel), whereas the CD25- T cells do not express Ep-CAM (data not shown). Flow cytometry was performed on a BD FACSCalibur™ (BD  Biosciences, San Jose, CA)

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