BD Pharmingen- FITC Mouse Anti-Human Syk_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
Syk
宿主
Mouse IgG2a, κ
免疫原
Human Syk peptide aa. 314-339
简单描述
Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igα, TCRζ, CD3ε, FcεRIβ, and FceRIγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cγ1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa; Accession number P43405).
商品描述
4D10
Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igα, TCRζ, CD3ε, FcεRIβ, and FceRIγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cγ1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa; Accession number P43405).
同种型
Mouse IgG2a, κ
克隆号
克隆 4D10 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
Syk
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Chu DH, van Oers NS, Malissen M, Harris J, Elder M, Weiss A. Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression. J Immunol. 1999; 163(5):2610-2620. (Immunogen: Flow cytometry, Western blot).
2. Costello PS, Turner M, Walters AE, et al. Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells. Oncogene. 1996; 13(12):2595-2605. (Biology).
3. Latour S, Veillette A. Proximal protein tyrosine kinases in immunoreceptor signaling. Curr Opin Immunol. 2001; 13:299-306. (Biology).
4. Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL. Tyrosine kinase SYK; essential functions for immunoreceptor signaling. Immunol Today. 2000; 21:148-154. (Biology).
参考图片
Flow cytometric analysis of Syk. Raji cells (1 x 10e6 cells/ml) were fixed and permeabilized using BD Cytofix/Cytoperm™ (30 minutes at room temperature). Cells were then stained (1 µg/ml for 1 hr at RT) with either a FITC-conjugated mouse Syk antibody (Cat. No. 552476) or a FITC conjugated mouse isotype control. Cells were washed and analyzed by flow cytometry.
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