CD126

Mouse IgG1, κ

Mixture of U266, XG-1and BWD41 cells

The M182 antibody reacts with the a subunit of the human IL-6 Receptor (IL-6Rα, aka, CD126). The human IL-6Rα is an 80 kDa type I transmembrane glycoprotein, also known as B cell stimulatory factor-2 (BSF-2) receptor and IL-6 receptor. The IL-6Rα subunit associates with the 130-160 kDa gp130 subunit (IL-6 receptor ß chain, CD130), that is shared with the receptors for Leukemia Inhibitory Factor (LIF), Ciliary Neurotropic Factor (CNTF), Oncostatin M (OSM), IL-11, Cardiotropin 1 (CT-1) and possibly Neurotrophin-1/B Cell-Stimulating Factor 3 (NNT-1/BSF-3). The IL-6Rα chain binds IL-6 with low affinity, however the association with CD130 stabilizes the IL-6/IL-6Rα complex resulting in the formation of a high affinity complex. The IL-6R ß chain mediates signal transduction. IL-6Rα's are expressed at high levels by activated and EBV-transformed B cells, plasma cells and myeloma cells and at lower levels by most leucocytes, epithelial cells, fibroblasts, hepatocytes and neural cells. IL-6Rα exists in soluble form in human serum. The serum levels of soluble IL-6Raappear to elevate in pathological situations such as multiple myeloma, Grave's disease, juvenile chronic arthritis and HIV.10-13 The immunogen used to generate the M182 hybridoma was a mixture of U266, XG-1 (human myeloma cell line expressing membrane IL-6R) and BWD41 cells (murine thymoma cell line transfected with cDNA encoding the extracellular part of IL-6Rα).

M182 The M182 antibody reacts with the a subunit of the human IL-6 Receptor (IL-6Rα, aka, CD126). The human IL-6Rα is an 80 kDa type I transmembrane glycoprotein, also known as B cell stimulatory factor-2 (BSF-2) receptor and IL-6 receptor. The IL-6Rα subunit associates with the 130-160 kDa gp130 subunit (IL-6 receptor ß chain, CD130), that is shared with the receptors for Leukemia Inhibitory Factor (LIF), Ciliary Neurotropic Factor (CNTF), Oncostatin M (OSM), IL-11, Cardiotropin 1 (CT-1) and possibly Neurotrophin-1/B Cell-Stimulating Factor 3 (NNT-1/BSF-3). The IL-6Rα chain binds IL-6 with low affinity, however the association with CD130 stabilizes the IL-6/IL-6Rα complex resulting in the formation of a high affinity complex. The IL-6R ß chain mediates signal transduction. IL-6Rα's are expressed at high levels by activated and EBV-transformed B cells, plasma cells and myeloma cells and at lower levels by most leucocytes, epithelial cells, fibroblasts, hepatocytes and neural cells. IL-6Rα exists in soluble form in human serum. The serum levels of soluble IL-6Raappear to elevate in pathological situations such as multiple myeloma, Grave's disease, juvenile chronic arthritis and HIV.10-13 The immunogen used to generate the M182 hybridoma was a mixture of U266, XG-1 (human myeloma cell line expressing membrane IL-6R) and BWD41 cells (murine thymoma cell line transfected with cDNA encoding the extracellular part of IL-6Rα).

Mouse IgG1, κ

克隆 M182 (RUO)

0.5 mg/ml

ELISA Detection (Routinely Tested)

Human (QC Testing)

CD126 (IL-6 Receptor)

IL-6 Receptor α Chain

Aqueous buffered solution containing ≤0.09% sodium azide.

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