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BD IMag- Anti-Human CD3 Magnetic Particles - DM_BD Pharmingen

产品信息
抗原名称
CD3
宿主
Mouse IgG2a, κ
简单描述
BD IMag™ Anti-Human CD3 Particles DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD3-bearing leukocytes using the BD IMag™ Cell Separation Magnet. CD3 has been reported to be expressed on all mature T lymphocytes and on developing thymocytes.
商品描述
HIT3a BD IMag™ Anti-Human CD3 Particles DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD3-bearing leukocytes using the BD IMag™ Cell Separation Magnet. CD3 has been reported to be expressed on all mature T lymphocytes and on developing thymocytes.
同种型
Mouse IgG2a, κ
克隆号
克隆 HIT3a (RUO)
应用
实验应用
Cell separation (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD3
背景
别名
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 2. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050. 3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

参考图片

Positive selection and depletion of human CD3-positive PBMC. Leukocytes were labeled with BD IMag™ anti-human CD3 Particles - DM (Cat. No. 552593) as described in the protocol. After labeling, the cells were separated using the BD IMag™ Cell Separation Magnet (Cat. No. 552311), and the negative (CD3-) and positive (CD3+) fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh PBMC (left panel), the negative fraction (middle panel), and the positive fraction (right panel) were stained with APC Anti-Human CD3 (Cat. No. 555335). The percent CD3+ cells in each sample is given.

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