BD Pharmingen- Purified Mouse Anti-Pig CD8b_BD Pharmingen
产品信息
抗原名称
CD8b
宿主
Mouse BALB/c IgG2a, κ
免疫原
Pig thymocytes
简单描述
The 295/33-25 monoclonal antibody specifically binds to an epitope on the CD8α chain, a 35 kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and a β chain. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4-negative CTL effectors/precursors and CD8-dull CD4-positive T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1; but not CD3, CD4, CD5, or CD6. mAb 295/33-25 was clustered as anti-CD8b at the First International Swine CD workshop.
商品描述
295/33-25
The 295/33-25 monoclonal antibody specifically binds to an epitope on the CD8α chain, a 35 kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and a β chain. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4-negative CTL effectors/precursors and CD8-dull CD4-positive T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1; but not CD3, CD4, CD5, or CD6. mAb 295/33-25 was clustered as anti-CD8b at the First International Swine CD workshop.
同种型
Mouse BALB/c IgG2a, κ
克隆号
克隆 295/33-25 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Cytotoxicity, ELISA, Immunohistochemistry-frozen, Immunoprecipitation (Reported), Immunohistochemistry-paraffin (Not Recommended)
反应种属
Pig (QC Testing)
目标/特异性
CD8
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(10)
1. Dato ME, Kim YB. Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells. J Immunol. 1990; 144(11):4452-4462. (Biology).
2. Jonjic N, Jonjic S, Saalmuller A, Rukavina D, Koszinowski UH. Distribution of T-lymphocyte subsets in porcine lymphoid tissues. Immunology. 1987; 60(3):395-401. (Clone-specific: Immunohistochemistry).
3. Jonjic S, Koszinowski UH. Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine. J Immunol. 1984; 133(2):647-652. (Immunogen: Cytotoxicity, ELISA, Immunoprecipitation).
4. Pescovitz MD, Lowman MA, Sachs DH. Expression of T-cell associated antigens by porcine natural killer cells. Immunology. 1988; 65(2):267-271. (Biology).
5. Saalmuller A, Aasted B, Canals A, et al. Analyses of mAb reactive with porcine CD8. Vet Immunol Immunopathol. 1994; 43(1-3):249-254. (Clone-specific: Immunoprecipitation).
6. Saalmuller A, Hirt W, Maurer S, Weiland E. Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen. Immunology. 1994; 81(4):578-583. (Biology).
7. Saalmuller A, Pauly T, Hohlich BJ, Pfaff E. Characterization of porcine T lymphocytes and their immune response against viral antigens. J Biotechnol. 1999; 73(2-3):223-233. (Biology).
8. Saalmuller A, Werner T, Fachinger V. T-helper cells from naive to committed. Vet Immunol Immunopathol. 2002; 87(3-4):137-145. (Biology).
9. Zuckermann FA, Pescovitz MD, Aasted B, et al. Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop. Vet Immunol Immunopathol. 1998; 60(3-4):291-303. (Biology).
10. Zuckermann FA. Extrathymic CD4/CD8 double positive T cells. Vet Immunol Immunopathol. 1999; 72(1-2):55-66. (Biology).
参考图片
CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390), then PE-conjugated mAb 74-12-4 (anti-pig CD4a, Cat. No. 559586). Erythrocytes were lysed (BD PharmLyse™, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.
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