BD Pharmingen- Purified Rat Anti-Human CD29_BD Pharmingen
产品信息
抗原名称
CD29 (Integrin β1)
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
免疫原
Purified Fibronectin Receptor from Human Placenta
简单描述
The Mab 13 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The family of β1 integrins includes receptors for vascular cell adhesion molecule 1 (VCAM-1), extracellular matrix (ECM) components such as collagen (COL), fibronectin (FN), laminin (LM) and vitronectin (VN), and pathogens. The β1 subunit is widely expressed by T and B cells, dendritic cells, NK cells, monocytes and macrophages, granulocytes, platelets, endothelial and epithelial cells, and stem cells.
商品描述
Mab 13
The Mab 13 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The family of β1 integrins includes receptors for vascular cell adhesion molecule 1 (VCAM-1), extracellular matrix (ECM) components such as collagen (COL), fibronectin (FN), laminin (LM) and vitronectin (VN), and pathogens. The β1 subunit is widely expressed by T and B cells, dendritic cells, NK cells, monocytes and macrophages, granulocytes, platelets, endothelial and epithelial cells, and stem cells.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
克隆号
克隆 Mab 13 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), ELISA, Functional assay, Immunoprecipitation, Western blot (Reported)
反应种属
Human (QC Testing)
目标/特异性
CD29 (Integrin β1)
背景
别名
ITGB1; Integrin beta-1; Integrin β1; FNRB; GPIIA; ITB1; MDF2; MSK12; VLA-4β
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(14)
1. Akiyama SK, Larjava H, Yamada KM. Differences in the biosynthesis and localization of the fibronectin receptor in normal and transformed cultured human cells. Cancer Res. 1990 March; 50(50):1601-1607. (Biology).
2. Akiyama SK, Yamada SS, Chen WT, Yamada KM. Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly, and cytoskeletal organization. J Cell Biol. 1989 August; 109(2):863-875. (Immunogen).
3. Bohnsack JF. CD11/CD18-independent neutrophil adherence to laminin is mediated by the integrin VLA-6. Blood. 1992 March; 79(6):1545-1552. (Biology).
4. Chan BM, Elices MJ, Murphy E, Hemler ME. Adhesion to vascular cell adhesion molecule 1 and fibronectin. Comparison of alpha 4 beta 1 (VLA-4) and alpha 4 beta 7 on the human B cell line JY. J Biol Chem. 1992 April; 267(12):8366-8370. (Biology).
5. Ennis E, Isberg RR, Shimizu Y. Very late antigen 4-dependent adhesion and costimulation of resting human T cells by the bacterial beta 1 integrin ligand invasin. J Exp Med. 1993 January; 177(1):207-212. (Biology).
6. Guo M, Kim LT, Akiyama SK, Gralnick HR, Yamada KM, Grinnell F. Altered processing of integrin receptors during keratinocyte activation. Exp Cell Res. 1991 August; 195(2):315-322. (Biology).
7. Hemler ME, Bodorova J, Kawaguchi S, Weitzman J, Kassner P. Adhesion structures subpanel 6, β1 integrins/VLA: CD29/CD49. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1609-1612.
8. Hertle MD, Adams JC, Watt FM. Integrin expression during human epidermal development in vivo and in vitro. Development. 1991 May; 112(1):193-206. (Biology).
9. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992; 69(1):11-25. (Biology).
10. Larjava H, Peltonen J, Akiyama SK, et al. Novel function for beta 1 integrins in keratinocyte cell-cell interactions. J Cell Biol. 1990 March; 110(3):803-815. (Biology).
11. Massia SP, Hubbell JA. Vascular endothelial cell adhesion and spreading promoted by the peptide REDV of the IIICS region of plasma fibronectin is mediated by integrin alpha 4 beta 1. J Biol Chem. 1992 July; 267(20):14019-14026. (Biology).
12. Peltonen J, Larjava H, Jaakkola S, et al. Localization of integrin receptors for fibronectin, collagen, and laminin in human skin. Variable expression in basal and squamous cell carcinomas. J Clin Invest. 1989 December; 84(6):1916-1923. (Biology).
13. Rodriguez RM, Pitzalis C, Kingsley GH, Henderson E, Humphries MJ, Panayi GS. T lymphocyte adhesion to fibronectin (FN): a possible mechanism for T cell accumulation in the rheumatoid joint. Clin Exp Immunol. 1992 September; 89(3):439-445. (Biology).
14. Teixidó J, Hemler ME, Greenberger JS, Anklesaria P. Role of beta 1 and beta 2 integrins in the adhesion of human CD34hi stem cells to bone marrow stroma. J Clin Invest. 1992 August; 90(2):358-367. (Biology).
参考图片
Flow cytometric analysis of CD29 expression on human peripheral blood lymphocytes. Whole blood was stained with Purified Rat Anti-Human CD29 antibody (Cat. No. 552828; solid line histogram) or with Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927/555841; dashed line histogram), followed by FITC Goat Anti-Rat Ig (Cat. No 554016). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Flow cytometric analysis of CD29 expression on human peripheral blood lymphocytes. Whole blood was stained with Purified Rat Anti-Human CD29 antibody (Cat. No. 552828; solid line histogram) or with Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927/555841; dashed line histogram), followed by FITC Goat Anti-Rat Ig (Cat. No 554016). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
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