BD Pharmingen- FITC Rat IgG1 - Isotype Control_BD Pharmingen
产品信息
荧光素标记
FITC
免疫原
Mouse immunoglobulin
简单描述
Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.
This reagent has been used as isotype control in immunophenotypic studies with baboon, rhesus, or cynomolgus peripheral blood cells.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
R3-34
Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.
This reagent has been used as isotype control in immunophenotypic studies with baboon, rhesus, or cynomolgus peripheral blood cells.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG1, κ
克隆号
克隆 R3-34 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
推荐用量
20 µl
目标/特异性
IgG1 Isotype Control
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
Profile of intracellular staining of rat IgG1 isotype control (R3-34) on permeabilized, SEB+CD28/49d-activated, CD3+ peripheral blood lymphocytes of cynomolgus macaque (Macaca mulatta) monkey analyzed by flow cytometry
Profile of intracellular staining of rat IgG1 isotype control (R3-34) on permeabilized, SEB+CD28/49d-activated, CD3+ peripheral blood lymphocytes of cynomolgus macaque (Macaca mulatta) monkey analyzed by flow cytometry
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