BD Pharmingen- FITC Rat Anti-Mouse CD45_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
CD45
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
免疫原
Mouse Thymus / Spleen
简单描述
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.
商品描述
30-F11
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
克隆号
克隆 30-F11 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD45
背景
别名
Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
2. Lagasse E, Connors H, Al-Dhalimy M, et al. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nat Med. 2000; 6(11):1212-1213. (Biology).
3. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979; 47:63-90. (Immunogen).
4. Thomas ML. The leukocyte common antigen family. Annu Rev Immunol. 1989; 7:339-369. (Biology).
数据库链接
Entrez-Gene ID
19264
参考图片
Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either FITC Rat IgG2b, κ Isotype Control (Cat. No. 553988; dashed line histogram) or FITC Rat Anti-Mouse CD45 antibody (Cat. No. 553079/ 561088/ 553080; solid line histogram) at 0.15 µg/test. The fluorescence histogram showing CD45 fluorescence (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System X-20 and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
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