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BD Pharmingen- FITC Rat Anti-Mouse CD11a_BD Pharmingen

艾美捷科技 • 2025年08月20日 16:08:05 • 抗体 • 阅读 12 • 标签
产品信息
荧光素标记
FITC
抗原名称
CD11a
宿主
Rat IgG2a, κ
免疫原
Not Reported
简单描述
The 2D7 monoclonal antibody specifically binds to the 180-kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8b- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intracellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb 2D7 has been reported to block an in vitro allogeneic mixed-leukocyte reaction. The 2D7 and M17/4 (Cat. No. 553337, for the NA/LE™ format) antibodies are reported to recognize different epitopes of the CD11a molecule.
商品描述
2D7 The 2D7 monoclonal antibody specifically binds to the 180-kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8b- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intracellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb 2D7 has been reported to block an in vitro allogeneic mixed-leukocyte reaction. The 2D7 and M17/4 (Cat. No. 553337, for the NA/LE™ format) antibodies are reported to recognize different epitopes of the CD11a molecule.
同种型
Rat IgG2a, κ
克隆号
克隆 2D7 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
Ly-6A/E (Sca-1)
背景
别名
Itgal; Integrin alpha-L; Integrin αL; ITAL; LFA-1A; LFA-1α; Ly-15
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Huleatt JW, Lefrancois L. Beta2 integrins and ICAM-1 are involved in establishment of the intestinal mucosal T cell compartment. Immunity. 1996; 5(3):263-273. (Biology). 2. Kootstra CJ, Van Der Giezen DM, Van Krieken JH, De Heer E, Bruijn JA. Effective treatment of experimental lupus nephritis by combined administration of anti-CD11a and anti-CD54 antibodies. Clin Exp Immunol. 1997; 108(2):324-332. (Biology). 3. Larson RS, Springer TA. Structure and function of leukocyte integrins. Immunol Rev. 1990; 114:181-217. (Biology). 4. Masten BJ, Yates JL, Pollard Koga AM, Lipscomb MF. Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L. Am J Respir Cell Mol Biol. 1997; 16(3):335-342. (Clone-specific: Blocking, Flow cytometry). 5. Potter TA, Grebe K, Freiberg B, Kupfer A. Formation of supramolecular activation clusters on fresh ex vivo CD8+ T cells after engagement of the T cell antigen receptor and CD8 by antigen-presenting cells. Proc Natl Acad Sci U S A. 2001; 98(22):12624-12629. (Clone-specific: Immunofluorescence). 6. Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-314. (Biology).
数据库链接
Entrez-Gene ID
16408

参考图片

Multiparameter flow cytometric analysis of CD11a expression on mouse bone-marrow cells. BALB/c mouse bone-marrow cells were stained with either FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; dashed line histograms) or FITC Rat Anti-Mouse CD11a (Cat. No. 553120/561683; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphoid (Left Panel) or myeloid (Right Panel) cells.

Multiparameter flow cytometric analysis of CD11a expression on mouse bone-marrow cells. BALB/c mouse bone-marrow cells were stained with either FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; dashed line histograms) or FITC Rat Anti-Mouse CD11a (Cat. No. 553120/561683; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphoid (Left Panel) or myeloid (Right Panel) cells.

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