CD24

Rat LEW, also known as Lewis IgM, κ

C3H/HeJ mouse thymocytes

The J11d antibody reacts with CD24 (Heat Stable Antigen, HSA or HsAg), a variably glycosylated membrane protein found on erythrocytes, granulocytes, monocytes, and lymphocytes. Levels of expression of CD24 vary during differentiation of the T and B cell lineages: Immature B cells in the bone marrow and spleen of adult mice express high levels of CD24, whereas mature peripheral B cells express intermediate levels of CD24. Similarly, the majority of thymocytes express high levels of CD24, while mature thymic and peripheral T cells do not express CD24. Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24. CD24 is not expressed by NK cells. CD24 is a ligand of CD62P (P-selectin). In vitro treatment of splenocytes with J11d antibody plus complement reduces primary IgM responses and proliferative responses to LPS. While the monoclonal antibodies 30-F1 (Cat. no. 558777), M1/69 (Cat. no. 557436), and J11d all react with CD24, they show subtle differences in the level of staining of different cell populations. When possible, investigators should continue to use the same monoclonal antibody as used in previous studies.

The J11d antibody reacts with CD24 (Heat Stable Antigen, HSA or HsAg), a variably glycosylated membrane protein found on erythrocytes, granulocytes, monocytes, and lymphocytes. Levels of expression of CD24 vary during differentiation of the T and B cell lineages: Immature B cells in the bone marrow and spleen of adult mice express high levels of CD24, whereas mature peripheral B cells express intermediate levels of CD24. Similarly, the majority of thymocytes express high levels of CD24, while mature thymic and peripheral T cells do not express CD24. Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24. CD24 is not expressed by NK cells. CD24 is a ligand of CD62P (P-selectin). In vitro treatment of splenocytes with J11d antibody plus complement reduces primary IgM responses and proliferative responses to LPS. While the monoclonal antibodies 30-F1 (Cat. no. 558777), M1/69 (Cat. no. 557436), and J11d all react with CD24, they show subtle differences in the level of staining of different cell populations. When possible, investigators should continue to use the same monoclonal antibody as used in previous studies.

Rat LEW, also known as Lewis IgM, κ

J11d

0.5 mg/ml

Flow cytometry (Routinely Tested) Immunohistochemistry-frozen (Tested During Development) Western blot, Depletion (Reported)

Mouse (QC Testing)

Heat Stable Antigen

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(11) 1. Aigner S, Ruppert M, Hubbe M, et al. Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int Immunol. 1995; 7(10):1557-1565. (Biology). 2. Allman DM, Ferguson SE, Lentz VM, Cancro MP. Peripheral B cell maturation. II. Heat-stable antigen(hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells. J Immunol. 1993; 151(9):4431-4444. (Biology). 3. Alterman LA, Crispe IN, Kinnon C. Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies. Eur J Immunol. 1990; 20(7):1597-1602. (Clone-specific: Western blot). 4. Bruce J, Symington FW, McKearn TJ, Sprent J. A monoclonal antibody discriminating between subsets of T and B cells. J Immunol. 1981; 127(6):2496-2501. (Immunogen: Depletion). 5. Crispe IN, Bevan MJ. Expression and functional significance of the J11d marker on mouse thymocytes. J Immunol. 1987 April; 138(7):2013-2018. (Biology). 6. Crowley M, Inaba K, Witmer-Pack M, Steinman RM. The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus. Cell Immunol. 1989; 118(1):108-125. (Biology). 7. Reichlin A, Iizuka K, Yokoyama WM. Isolation of murine natural killer cells. In: Coligan J, Kruisbeek AM, Margulies D, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1999:3.22.1-3.22.6. 8. Stall AM, Wells SM. FACS analysis of murine B-cell populations. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology. Blackwell Science Publishers; 1997:63.1-63.17. 9. Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Biology). 10. Wenger RH, Rochelle JM, Seldin MF, Kohler G, Nielsen PJ. The heat stable antigen (mouse CD24) gene is differentially regulated but has a housekeeping promoter. J Biol Chem. 1993; 268(31):23345-23352. (Clone-specific). 11. Woo J, Lu L, Rao AS, et al. Isolation, phenotype, and allostimulatory activity of mouse liver dendritic cells. Transplantation. 1994; 58(4):484-491. (Biology).