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BD Pharmingen- Biotin Hamster Anti-Mouse TCR - Chain_BD Pharmingen

艾美捷科技 • 2025年08月20日 16:43:16 • 抗体 • 阅读 9 • 标签
产品信息
抗原名称
TCR β Chain
宿主
Armenian Hamster IgG2, λ1
免疫原
TCR affinity-purified from mouse T-cell hybridoma DO-11.10
简单描述
The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.
商品描述
H57-597 The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.
同种型
Armenian Hamster IgG2, λ1
克隆号
克隆 H57-597 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
TCR β Chain
背景
别名
Tcrb; TCRbeta; TCRβ, T cell receptor beta chain
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(9) 1. Bendelac A, Killeen N, Littman DR, Schwartz RH. A subset of CD4+ thymocytes selected by MHC class I molecules. Science. 1994; 263(5154):1774-1778. (Biology). 2. Duke RC, Cohen JJ, Boehme SA, et al. Morphological, biochemical, and flow cytometric assays of apoptosis. In: Coligan J, Kruisbeek AM, Margulies D, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1995:3.17.1-3.17.33. 3. Gascoigne NR. Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990; 265(16):9296-9301. (Clone-specific: Immunoprecipitation). 4. Groettrup M, von Boehmer H. T cell receptor beta chain dimers on immature thymocytes from normal mice. Eur J Immunol. 1993; 23(6):1393-1396. (Biology). 5. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Stimulation). 6. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Immunogen). 7. Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991; 147(6):1746-1751. (Biology). 8. Saint-Ruf C, Ungewiss K, Groettrup M, Bruno L, Fehling HJ, von Boehmer H. Analysis and expression of a cloned pre-T cell receptor gene. Science. 1994; 266(5188):1208-1212. (Clone-specific: Stimulation). 9. Vicari AP, Zlotnik A. Mouse NK1.1+ T cells: a new family of T cells. Immunol Today. 1996; 17(2):71-76. (Biology).
数据库链接
Entrez-Gene ID
21577

参考图片

Flow cytometric analysis of αβ TCR expression in spleen and thymus. BALB/c splenocytes (left panels) were simultaneously stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033), and either Biotin Hamster IgG2, λ1 Isotype Control (Cat. no. 553963; top left panel) or Biotin Hamster Anti-Mouse TCR β Chain (Cat. No. 553168; bottom left panel) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). BALB/c thymocytes (right panels) were stained with either Biotin Hamster IgG2, λ1 Isotype Control (top right panel) or Biotin Hamster Anti-Mouse TCR β Chain (bottom right panel), followed by Avidin FITC. The fluorescence histograms and contour plots were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.

Flow cytometric analysis of αβ TCR expression in spleen and thymus. BALB/c splenocytes (left panels) were simultaneously stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033), and either Biotin Hamster IgG2, λ1 Isotype Control (Cat. no. 553963; top left panel) or Biotin Hamster Anti-Mouse TCR β Chain (Cat. No. 553168; bottom left panel) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). BALB/c thymocytes (right panels) were stained with either Biotin Hamster IgG2, λ1 Isotype Control (top right panel) or Biotin Hamster Anti-Mouse TCR β Chain (bottom right panel), followed by Avidin FITC. The fluorescence histograms and contour plots were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.

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