BD Pharmingen- FITC Rat Anti-Mouse V- 6 T-Cell Receptor_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
TCR Vβ6
宿主
Rat F344, also known as Fischer, CDF IgG2b, λ
免疫原
C57BL/6 Mouse Helper T-Cell Clone OI11
简单描述
The RR4-7 antibody specifically reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the
a
(
e.g.,
C57BR, C57L, SJL) and
b
(
e.g.,
A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the
Tcrb
gene complex. The
Tcrb-V6
gene locus is deleted in mice having the
c
(
e.g.,
RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by
Mtv-7
(
Mls-1[a], Mls[a]
) endogenous provirus (
e.g.,
AKR, CBA/J, C58, DBA/2, NZB), or
Mtv
-43 endogenous provirus (
e.g.,
MA/MyJ). Exogenous MMTV-SW, as well as endogenous
Mtv
-44-encoded superantigen (
e.g.,
NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks
in vitro
proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells.
商品描述
RR4-7
The RR4-7 antibody specifically reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the
a
(
e.g.,
C57BR, C57L, SJL) and
b
(
e.g.,
A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the
Tcrb
gene complex. The
Tcrb-V6
gene locus is deleted in mice having the
c
(
e.g.,
RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by
Mtv-7
(
Mls-1[a], Mls[a]
) endogenous provirus (
e.g.,
AKR, CBA/J, C58, DBA/2, NZB), or
Mtv
-43 endogenous provirus (
e.g.,
MA/MyJ). Exogenous MMTV-SW, as well as endogenous
Mtv
-44-encoded superantigen (
e.g.,
NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks
in vitro
proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells.
同种型
Rat F344, also known as Fischer, CDF IgG2b, λ
克隆号
克隆 RR4-7 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry/immunoassay (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ6
背景
别名
TCR V beta 6; TCR Vβ6
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(14)
1. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology).
2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology).
3. Held W, Shakhov AN, Waanders G, et al. An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7). J Exp Med. 1992; 175(6):1623-1633. (Biology).
4. Jones LA, Chin LT, Longo DL, Kruisbeek AM. Peripheral clonal elimination of functional T cells. Science. 1990; 250(4988):1726-1729. (Biology).
5. Jones LA, Chin LT, Merriam GR, Nelson LM, Kruisbeck AM. Failure of clonal deletion in neonatally thymectomized mice: tolerance is preserved through clonal anergy. J Exp Med. 1990; 172(5):1277-1285. (Biology).
6. Kanagawa O, Palmer E, Bill J. The T cell receptor V beta 6 domain imparts reactivity to the Mls-1a antigen. Cell Immunol. 1989; 119(2):412-426. (Immunogen: Blocking).
7. Kanagawa O. In vivo T cell tumor therapy with monoclonal antibody directed to the V beta chain of T cell antigen receptor. J Exp Med. 1989; 170(5):1513-1519. (Clone-specific).
8. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific).
9. Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology).
10. Rocha B, Vassalli P, Guy-Grand D. The V beta repertoire of mouse gut homodimeric alpha CD8+ intraepithelial T cell receptor alpha/beta + lymphocytes reveals a major extrathymic pathway of T cell differentiation. J Exp Med. 1991; 173(2):483-486. (Biology).
11. Rudy CK, Kraus E, Palmer E, Huber BT. Mls-1-like superantigen in the MA/MyJ mouse is encoded by a new mammary tumor provirus that is distinct from Mtv-7. J Exp Med. 1992; 175(6):1613-1621. (Biology).
12. Tomonari K, Fairchild S. Positive and negative selection of Tcrb-V6+ T cells. Immunogenetics. 1992; 36(4):230-237. (Biology).
13. Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991; 21(4):1007-1011. (Clone-specific).
14. Webb S, Morris C, Sprent J. Extrathymic tolerance of mature T cells: clonal elimination as a consequence of immunity. Cell. 1990; 63(6):1249-1256. (Biology).
数据库链接
Entrez-Gene ID
110317
参考图片
Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553193), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.
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