产品信息
荧光素标记
PE
抗原名称
CD71 (Transferrin Receptor)
宿主
Rat WF, also known as Wistar Furth IgG1, κ
免疫原
Mouse cell line
简单描述
The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.
商品描述
C2
The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.
同种型
Rat WF, also known as Wistar Furth IgG1, κ
克隆号
克隆 C2 (also known as C2F2) (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD71 (Transferrin Receptor)
背景
别名
Transferrin Receptor; TR; TfR; TfR1; Tfrc; Trfr; Mtvr-1
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Activation).
2. Kemp JD, Thorson JA, McAlmont TH, Horowitz M, Cowdery JS, Ballas ZK. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol. 1987; 138(8):2422-2426. (Immunogen: Activation).
3. Lok CN, Loh TT. Regulation of transferrin function and expression: review and update. Biol Signals Recept. 1998; 7(3):157-178. (Biology).
4. Thorson JA, Smith KM, Gomez F, Naumann PW, Kemp JD. Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG Mabs against the transferrin receptor and the iron chelator deferoxamine. Cell Immunol. 1991; 134(1):126-137. (Clone-specific: Activation).
数据库链接
Entrez-Gene ID
22042
参考图片
Two-color analysis of the expression of CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with biotinylated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553672) and PE-conjugated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Two-color analysis of the expression of CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with biotinylated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553672) and PE-conjugated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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