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BD Pharmingen- PE Rat Anti-Mouse CD43_BD Pharmingen

产品信息
荧光素标记
PE
抗原名称
CD43 (Leukosialin)
宿主
Rat DA x LOU IgG2a, κ
免疫原
Mouse Plasmacytoma MOPC-315
简单描述
The S7 monoclonal antibody specifically binds to the 115 kDa glycosylated form of CD43 (Ly-48, Leukosialin). CD43 is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion.
商品描述
S7 The S7 monoclonal antibody specifically binds to the 115 kDa glycosylated form of CD43 (Ly-48, Leukosialin). CD43 is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion.
同种型
Rat DA x LOU IgG2a, κ
克隆号
克隆 S7 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD43
背景
别名
Spn; Sialophorin; Leukosialin; Ly-48; Ly48; Galgp; LEUK
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.

参考图片

Flow cytometric analysis of CD43 expression on mouse splenocytes. BALB/c splenocytes were stained with either PE Rat Anti-Mouse CD43 (Cat. No. 553271/561857; solid line histogram) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram). Fluorescence histograms depicting CD43 (or Ig isotype control) expression were derived from side and forward light-scatter characteristics of viable splenocytes.

Flow cytometric analysis of CD43 expression on mouse splenocytes. BALB/c splenocytes were stained with either PE Rat Anti-Mouse CD43 (Cat. No. 553271/561857; solid line histogram) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram). Fluorescence histograms depicting CD43 (or Ig isotype control) expression were derived from side and forward light-scatter characteristics of viable splenocytes.

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