BD Pharmingen- PE Hamster Anti-Mouse CD28_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD28
宿主
Syrian Hamster IgG2, λ1
免疫原
Mouse EL-4 (T-cell lymphoma) Cells
简单描述
The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor; its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.
商品描述
37.51
The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor; its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.
同种型
Syrian Hamster IgG2, λ1
克隆号
克隆 37.51 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
CD28
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
12487
参考图片
Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 [Mouse BD Fc Block™] (Cat. No. 553141), BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. Mo. 553046) and FITC-conjugated anti-mouse CD8 mAb 53-6.7 (Cat. No. 553030) in addition to staining with PE conjugated anti-mouse CD28 clone 37.51 (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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