Flow cytometric analysis of CD11b expression on mouse bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-CD11b antibody (Cat. No. 553308/550282/557394; right panel), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). The contour plots showing CD11b expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of myeloid cells (ie, moderate-to-high side light-scatter-gated events). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Flow cytometric analysis of CD11b expression on mouse bone-marrow myeloid cells.  BALB/c bone-marrow leukocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-CD11b antibody (Cat. No. 553308/550282/557394; right panel), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). The contour plots showing CD11b expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of myeloid cells (ie, moderate-to-high side light-scatter-gated events). Flow cytometry was performed on a BD FACScan™ flow cytometry system.