CD49e (Integrin α5)

Rat LEW, also known as Lewis IgG2a, κ

Mouse Mast Cell Line MC/9

The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized 5H10-27 (MFR5) antibody reportedly costimulates the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

5H10-27 (MFR5) The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized 5H10-27 (MFR5) antibody reportedly costimulates the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Rat LEW, also known as Lewis IgG2a, κ

克隆 5H10-27 (MFR5) (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), (Co)-stimulation, Blocking, Immunofluorescence, Immunohistochemistry-frozen (Reported), Immunohistochemistry-paraffin (Not Recommended)

Mouse (QC Testing)

CD49e (Integrin α5)

Itga5; ITA5; Integrin alpha-5; Fibronectin receptor alpha; Fnra; VLA5 alpha

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(10) 1. Cukierman E, Pankov R, Stevens DR, Yamada KM. Taking cell-matrix adhesions to the third dimension. Science. 2001; 294(5547):1708-1712. (Clone-specific: Immunofluorescence, Immunohistochemistry). 2. Gomez M, Cano A. Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells. Mol Carcinog. 1995; 12(3):153-165. (Clone-specific: Immunofluorescence, Immunohistochemistry). 3. Halvorson MJ, Coligan JE. Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes. J Immunol. 1995; 155(10):4567-4574. (Clone-specific: Blocking). 4. Hardy CL, Minguell JJ. Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3. J Cell Physiol. 1995; 164(2):315-323. (Biology). 5. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994; 20(1):25-44. (Immunogen: Blocking). 6. Rich S, Van Nood N, Lee HM. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996; 157(7):2916-2923. (Clone-specific: Blocking, (Co)-stimulation). 7. Ruppert M, Aigner S, Hubbe M, Yagita H, Altevogt P. The L1 adhesion molecule is a cellular ligand for VLA-5. J Cell Biol. 1995; 131(6):1881-1891. (Clone-specific: Blocking). 8. Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem. 1995; 270(19):11522-11531. (Clone-specific: Blocking). 9. Uhlenkott CE, Huijzer JC, Cardeiro DJ, Elstad CA, Meadows GG. Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine. Clin Exp Metastasis. 1996; 14(2):125-137. (Clone-specific: Blocking). 10. Yang JT, Hynes RO. Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins. Mol Biol Cell. 1996; 7(11):1737-1748. (Clone-specific: Immunofluorescence, Immunohistochemistry).