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BD Pharmingen- FITC Rat Anti-Mouse IgG2a-2b_BD Pharmingen

艾美捷科技 • 2025年08月21日 10:26:47 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
FITC
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled Mouse Ig
简单描述
The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes.
商品描述
R2-40 The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 R2-40 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
IgG2a, b
背景
别名
IgG2a (Ighg2a; Igh-1); IgG2b (Ighg2b; Igh-3; gamma2b)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1) 1. Benson MJ, Dillon SR, Castigli E, et al. Cutting edge: the dependence of plasma cells and independence of memory B cells on BAFF and APRIL.. J Immunol. 2008; 180(6):3655-9. (Biology).
数据库链接
Entrez-Gene ID
380793,380793, 16016

参考图片

Flow cytometric analysis of intracellular mouse IgG2a/2b on hybridoma cells. Permeabilized cells were stained with FITC Rat Anti-Mouse IgG2a/2b (Cat. No. 553399; solid line histogram) or with FITC Rat IgG1, κ Isotype Control (Cat. No. 554684; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a FACSCalibur™.

Flow cytometric analysis of intracellular mouse IgG2a/2b on hybridoma cells. Permeabilized cells were stained with FITC Rat Anti-Mouse IgG2a/2b (Cat. No. 553399; solid line histogram) or with FITC Rat IgG1, κ Isotype Control (Cat. No. 554684; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a FACSCalibur™.

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