BD Pharmingen- Purified Rat Anti-Mouse IgE_BD Pharmingen
产品信息
抗原名称
IgE
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Mouse IgE (pooled)
简单描述
The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.
商品描述
R35-72
The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 R35-72 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgE
背景
别名
Igh-7; immunoglobulin heavy chain 7 (heavy chain of IgE)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Borges MS, Kumagai Y, Okumura K, Hirayama N, Ovary Z, Tada T. Allelic polymorphism of murine IgE controlled by the seventh immunoglobulin heavy chain allotype locus. Immunogenetics. 1981; 13(6):499-507. (Biology).
2. Driver DJ, McHeyzer-Williams LJ, Cool M, Stetson DB, McHeyzer-Williams MG. Development and maintenance of a B220- memory B cell compartment. J Immunol. 2001; 167(3):1393-1405. (Biology).
3. Kamijo S, Takeda H, Tokura T, et al. IL-33-Mediated Innate Response and Adaptive Immune Cells Contribute to Maximum Responses of Protease Allergen-Induced Allergic Airway Inflammation. J Immunol. 2013; 190(9):4489-4499. (Biology).
4. Liu FT, Albrandt K, Sutcliffe JG, Katz DH. Cloning and nucleotide sequence of mouse immunoglobulin epsilon chain cDNA. Proc Natl Acad Sci U S A. 1982; 79(24):7852-7856. (Biology).
5. Markowitz JS, Rogers PR, Grusby MJ, Parker DC, Glimcher LH. B lymphocyte development and activation independent of MHC class II expression.. J Immunol. 1993; 150(4):1223-33. (Biology).
6. Nishida Y, Kataoka T, Ishida N, et al. Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster. Proc Natl Acad Sci U S A. 1981; 78(3):1581-1585. (Biology).
数据库链接
Entrez-Gene ID
16020
参考图片
IgE standard curve obtained using Purified Rat Anti-Mouse IgE (Cat. No. 553413) at 2 µg/ml for capture and Biotin Rat Anti-Mouse IgE (Cat. No. 553419) at 2 µg/ml for detection of the mouse IgE standard (Cat. No. 557079).
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