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BD Pharmingen- FITC Rat Anti-Mouse IgG1_BD Pharmingen

艾美捷科技 • 2025年08月21日 10:47:14 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
FITC
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled Mouse IgG1
简单描述
The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.
商品描述
A85-1 The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 A85-1 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
IgG1
背景
别名
Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
16017

参考图片

Flow cytometric analysis of IgG1 expression in an antibody-secreting hybridoma cell line. Hybridoma cell line was stained with either FITC Rat Anti-Mouse IgG1 (Cat. No. 553443/562026; solid line histogram), or FITC Rat IgG1, κ Isotype Control (Cat. No. 554684; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a BD FACSCalibur™.

Flow cytometric analysis of IgG1 expression in an antibody-secreting hybridoma cell line. Hybridoma cell line was stained with either FITC Rat Anti-Mouse IgG1 (Cat. No. 553443/562026; solid line histogram), or FITC Rat IgG1, κ Isotype Control (Cat. No. 554684; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a BD FACSCalibur™.

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