BD Pharmingen- PE Mouse Anti-Mouse I-A-d_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
I-Ad
宿主
Mouse SJL IgG2b, κ
免疫原
BALB/c mouse splenocytes
简单描述
The AMS-32.1 antibody specifically reacts with the I-A[d] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
AMS-32.1
The AMS-32.1 antibody specifically reacts with the I-A[d] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Mouse SJL IgG2b, κ
克隆号
克隆 AMS-32.1 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
I-A[d]
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Immunogen: Flow cytometry).
2. Ridgway WM, Ito H, Fasso M, Yu C, Fathman CG. Analysis of the role of variation of major histocompatibility complex class II expression on nonobese diabetic (NOD) peripheral T cell response. J Exp Med. 1998; 188(12):2267-2275. (Biology).
3. Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific).
参考图片
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