BD Pharmingen- PE Mouse Anti-Mouse H-2K-d_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
H-2Kd
宿主
Mouse SJL IgG2a, κ
免疫原
BALB/c Mouse Cells
简单描述
The SF1-1.1 antibody reacts with the α3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g,
b, j, k, p, q, s, v
) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e).
商品描述
SF1-1.1
The SF1-1.1 antibody reacts with the α3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g,
b, j, k, p, q, s, v
) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e).
同种型
Mouse SJL IgG2a, κ
克隆号
克隆 SF1-1.1 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
H-2K[d]
背景
别名
H-2kd; H2kd; MHC class I H-2Kd
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Abastado JP, Casrouge A, Kourilsky P. Differential role of conserved and polymorphic residues of the binding groove of MHC class I molecules in the selection of peptides. J Immunol. 1993; 151(7):3569-3575. (Clone-specific).
2. Noun G, Reboul M, Abastado JP, Jaulin C, Kourilsky P, Pla M. Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles. J Immunol. 1996; 157(6):2455-2461. (Clone-specific).
3. Zhao Y, Iwata M. Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506. Int Immunol. 1995; 7(9):1387-1396. (Biology).
参考图片
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