BD Pharmingen- Purified Mouse Anti-Pig Monocyte-Granulocyte_BD Pharmingen
产品信息
抗原名称
Monocyte/Granulocyte
宿主
Mouse BALB/c IgG2b, κ
免疫原
dd miniature swine thymocytes
简单描述
The 74-22-15A (switch variant of 74-22-15) monoclonal antibody, an isotype class-switch variant of mAb 74-22-15, specifically binds to a 230-kDa protein expressed by most pig macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes. mAb 74-22-15A does not crossreact with human or bovine cells. This clone was clustered as anti-SWC3a at the First International Swine CD workshop.
商品描述
74-22-15A
The 74-22-15A (switch variant of 74-22-15) monoclonal antibody, an isotype class-switch variant of mAb 74-22-15, specifically binds to a 230-kDa protein expressed by most pig macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes. mAb 74-22-15A does not crossreact with human or bovine cells. This clone was clustered as anti-SWC3a at the First International Swine CD workshop.
同种型
Mouse BALB/c IgG2b, κ
克隆号
克隆 74-22-15A (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Depletion, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
反应种属
Pig (QC Testing)
目标/特异性
SWC3a (Monocyte, Granulocyte)
背景
别名
SWC3; CD172a
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(8)
1. Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry).
2. Blecha F, Kielian T, McVey DS. Workshop studies on monoclonal antibodies reactive against porcine myeloid cells. Vet Immunol Immunopathol. 1994; 43(1-3):269-272. (Biology).
3. Haverson K, Bailey M, Higgins VR, Bland PW, Stokes CR. Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J Immunol Methods. 1994; 170(2):233-245. (Biology).
4. McCullough KC, Schaffner R, Natale V, Kim YB, Summerfield A. Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages. Vet Immunol Immunopathol. 1997; 58(3-4):265-275. (Biology).
5. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984; 133(1):368-375. (Immunogen: Immunoprecipitation).
6. Summerfield A, Haverson K, Thacker E, McCullough KC. Differentiation of porcine myeloid bone marrow haematopoietic cell populations. Vet Immunol Immunopathol. 2001; 80(1-2):121-129. (Biology).
7. Sundt TM, Arn JS, Sachs DH. Patterns of T cell-accessory cell interaction in the generation of primary alloresponses in the pig. Transplantation. 1992; 54(5):911-916. (Clone-specific: Depletion).
8. Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology).
参考图片
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