产品信息
荧光素标记
PE
抗原名称
CD138
宿主
Rat F344, also known as Fischer, CDF IgG2a, κ
免疫原
NAMRU mouse mammary gland epithelial cell line NMuMG
简单描述
The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow, and antibody-secreting cells including plasma cells (but not mature peripheral B cells). It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.
商品描述
281-2
The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow, and antibody-secreting cells including plasma cells (but not mature peripheral B cells). It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.
同种型
Rat F344, also known as Fischer, CDF IgG2a, κ
克隆号
克隆 281-2 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD138 (Syndecan-1)
背景
别名
SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
20969
参考图片
Expression of CD138 on mouse bone-marrow B lymphocytes. C57BL/6 bone-marrow leukocytes were simultaneously stained with PE-conjugated 281-2 mAb (Right panel) and FITC-conjugated RA3-6B2 mAb (anti-CD45R/B220, Cat. No. 553087/553088, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Expression of CD138 on mouse bone-marrow B lymphocytes. C57BL/6 bone-marrow leukocytes were simultaneously stained with PE-conjugated 281-2 mAb (Right panel) and FITC-conjugated RA3-6B2 mAb (anti-CD45R/B220, Cat. No. 553087/553088, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
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