BD Pharmingen- PE Hamster Anti-Mouse CD152_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD152
宿主
Armenian Hamster IgG1, κ
免疫原
Mouse CTLA-4 IgG2a Fusion
简单描述
The UC10-4F10-11 monoclonal antibody specifically binds to CD152, which is also known as Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4). CD152 is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CD152 may play roles in induction and/or maintenance of immunological tolerance, regulation of protective immunity, and autoimmune responses, and regulation of some aspects of thymocyte maturation. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leucocytes.
商品描述
UC10-4F10-11
The UC10-4F10-11 monoclonal antibody specifically binds to CD152, which is also known as Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4). CD152 is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CD152 may play roles in induction and/or maintenance of immunological tolerance, regulation of protective immunity, and autoimmune responses, and regulation of some aspects of thymocyte maturation. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leucocytes.
同种型
Armenian Hamster IgG1, κ
克隆号
克隆 UC10-4F10-11 (also known as UC10-4F10; 4F10) (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
CD152 (CTLA-4)
背景
别名
Ctla4; CTLA-4; Cytotoxic T-lymphocyte-associated protein 4; Ly-56
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(11)
1. Alegre ML, Noel PJ, Eisfelder BJ, et al. Regulation of surface and intracellular expression of CTLA4 on mouse T cells. J Immunol. 1996; 157(11):4762-4770. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Intracellular Staining/Flow Cytometry).
2. Cilio CM, Daws MR, Malashicheva A, Sentman CL, Holmberg D. Cytotoxic T lymphocyte antigen 4 is induced in the thymus upon in vivo activation and its blockade prevents anti-CD3-mediated depletion of thymocytes. J Exp Med. 1998; 188(7):1239-1246. (Clone-specific: Blocking, Intracellular Staining/Flow Cytometry).
3. Issazadeh S, Zhang M, Sayegh MH, Khoury SJ.. Acquired thymic tolerance: role of CTLA4 in the initiation and maintenance of tolerance in a clinically relevant autoimmune disease model. J Immunol. 1999; 162(2):761-765. (Clone-specific: In vivo exacerbation).
4. Kearney ER, Walunas TL, Karr RW, et al. Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4. J Immunol. 1995; 155(3):1032-1036. (Clone-specific: In vivo exacerbation).
5. Lee KM, Chuang E, Griffin M, et al. Molecular basis of T cell inactivation by CTLA-4. Science. 1998; 282(5397):2263-2266. (Clone-specific: Immunoprecipitation).
6. McCoy K, Camberis M, Gros GL. Protective immunity to nematode infection is induced by CTLA-4 blockade. J Exp Med. 1997; 186(2):183-187. (Clone-specific: In vivo exacerbation).
7. Perkins D, Wang Z, Donovan C, et al. Regulation of CTLA-4 expression during T cell activation. J Immunol. 1996; 156(11):4154-4159. (Clone-specific: Flow cytometry).
8. Perrin PJ, Maldonado JH, Davis TA, June CH, Racke MK. CTLA-4 blockade enhances clinical disease and cytokine production during experimental allergic encephalomyelitis. J Immunol. 1996; 157(4):1333-1336. (Clone-specific: In vivo exacerbation).
9. Read S, Malmstrom V, Powrie F. Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation. J Exp Med. 2000; 192(2):295-302. (Clone-specific: Intracellular Staining/Flow Cytometry).
10. Takahashi T, Tagami T, Yamazaki S, et al. Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4. J Exp Med. 2000; 192(2):303-309. (Clone-specific: Flow cytometry, In vivo exacerbation).
11. Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation.. Immunity. 1994; 1(5):405-13. (Immunogen: Flow cytometry, Immunoprecipitation, Stimulation).
数据库链接
Entrez-Gene ID
12477
参考图片
Expression of CD152 on activated T lymphocytes. BALB/c splenocytes were left unactivated (left panel) or were activated for 48 hours with ConA (right panel). The cells were then stained with FITC Rat anti-Mouse CD8a mAb 53-6.7 (Cat. No. 553030/553031) and PE Hamster anti-Mouse CD152 (open histogram), or no PE conjugate (filled histogram), in the presence of Mouse BD Fc Block™ (Purified Rat anti-Mouse CD16/CD32, Cat. No. 553141/553142). Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).
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