CD249

Mouse Mus spretus, also known as STF Mus spretus IgG2a, κ

Abelson murine leukemia virus-transformed BALB/c mouse pre-B cell line 18.81

The BP-1 monoclonal antibody specifically recognizes CD249 which is also known as, 6C3/BP-1, Enpep, or Ly-51. CD249 is a glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus- transformed, in vitro and in vivo ). CD249 is a homodimeric cell-surface glycoprotein with 140-kDa subunits that has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoieses, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity.  Subsets of normal bone marrow pre-B and B lymphocytes express low levels of CD249 which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the CD249 molecule in the IL-7-driven proliferation of B cell precursors has been postulated.  However, B-cell abnormalities were not detected in CD249/Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express CD249.

BP-1 The BP-1 monoclonal antibody specifically recognizes CD249 which is also known as, 6C3/BP-1, Enpep, or Ly-51. CD249 is a glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus- transformed, in vitro and in vivo ). CD249 is a homodimeric cell-surface glycoprotein with 140-kDa subunits that has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoieses, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity.  Subsets of normal bone marrow pre-B and B lymphocytes express low levels of CD249 which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the CD249 molecule in the IL-7-driven proliferation of B cell precursors has been postulated.  However, B-cell abnormalities were not detected in CD249/Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express CD249.

Mouse Mus spretus, also known as STF Mus spretus IgG2a, κ

克隆 BP-1 (RUO)

0.2 mg/ml

Flow cytometry (Routinely Tested)

Mouse (QC Testing)

CD249

Bp-1/6C3; Ly51; Ly-51; Enpep; Glutamyl aminopeptidase; APA; EAP

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(14) 1. Cooper MD, Mulvaney D, Coutinho A, Cazenave PA. A novel cell surface molecule on early B-lineage cells. Nature. 1986; 321(6070):616-618. (Immunogen). 2. Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. J Exp Med. 1991; 173(5):1213-1225. (Biology). 3. Li L, Wu Q, Wang J, Bucy RP, Cooper MD. Widespread tissue distribution of aminopeptidase A, an evolutionarily conserved ectoenzyme recognized by the BP-1 antibody. Tissue Antigens. 1993; 42(5):488-496. (Biology). 4. Lin Q, Taniuchi I, Kitamura D. T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice. Int Immunol. 1998; 160(10):4681-4687. (Biology). 5. Ramakrishnan L, Wu Q, Yue A, Cooper MD, Rosenberg N. BP-1/6C3 expression defines a differentiation stage of transformed pre-B cells and is not related to malignant potential. J Immunol. 1990; 145(5):1603-1608. (Biology). 6. Sherwood PJ, Weissman IL. The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells. Int Immunol. 1990; 2(5):399-406. (Biology). 7. Vremec D, Shortman K. Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes. J Immunol. 1997; 159(2):565-573. (Biology). 8. Wang J, Cooper MD. Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity. Proc Natl Acad Sci U S A. 1990; 90(4):1222-1226. (Biology). 9. Welch PA, Burrows PD, Namen A, Gillis S, Cooper MD. Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth. Int Immunol. 1990; 2(8):697-705. (Biology). 10. Welch PA. Regulation of B cell precursor proliferation by aminopeptidase A. Int Immunol. 1995; 7(5):737-746. (Biology). 11. Wu L, Vremec D, Ardavin C, et al. Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation. Eur J Immunol. 1995; 25(2):418-425. (Biology). 12. Wu Q, Lahti JM, Air GM, Burrows PD, Cooper MD. Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family. Proc Natl Acad Sci U S A. 1990; 87(3):993-997. (Biology). 13. Wu Q, Li L, Cooper MD, Pierres M, Gorvel JP. Aminopeptidase A activity of the murine B-lymphocyte differentiation antigen BP-1/6C3. Proc Natl Acad Sci U S A. 1991; 88(2):676-680. (Clone-specific). 14. Wu Q, Tidmarsh GF, Welch PA, Pierce JH, Weissman IL, Cooper MD. The early B lineage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule. J Immunol. 1989; 143(10):3303-3308. (Biology).