CD35

Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ

Purified mouse CR1

The 8C12 antibody recognizes an epitope present on the 190-kDa complement  receptor protein, originally designated CR1 (CD35), but not the 145-150-kDa CR2 (CD21) molecule.  Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane  proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene.  Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively.  The epitope recognized by 8C12 mAb is only present on CD35/CD21b.   Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35).  In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N -formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets.  In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages.  The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes,  to block the complement-dependent trapping of immune complexes by follicular dendritic cells,  and to down-regulate mouse CD35 expression upon in vivo application,  inhibiting only some primary antibody responses to immunization.  B lymphocytes of Cr2 [ null ] mice display impaired humoral immune responses in vivo.  The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6, and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.

8C12 The 8C12 antibody recognizes an epitope present on the 190-kDa complement  receptor protein, originally designated CR1 (CD35), but not the 145-150-kDa CR2 (CD21) molecule.  Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane  proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene.  Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively.  The epitope recognized by 8C12 mAb is only present on CD35/CD21b.   Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35).  In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N -formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets.  In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages.  The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes,  to block the complement-dependent trapping of immune complexes by follicular dendritic cells,  and to down-regulate mouse CD35 expression upon in vivo application,  inhibiting only some primary antibody responses to immunization.  B lymphocytes of Cr2 [ null ] mice display impaired humoral immune responses in vivo.  The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6, and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.

Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ

克隆 8C12 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported)

Mouse (QC Testing)

CD35

CR1, CD21b

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(12) 1. Ahearn JM, Fischer MB, Croix D, et al. Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen. Immunity. 1996; 4(3):251-262. (Biology). 2. Fischer MB, Goerg S, Shen L, et al. Dependence of germinal center B cells on expression of CD21/CD35 for survival. Science. 1998; 280(5363):582-585. (Biology). 3. Fu YX, Huang G, Wang Y, Chaplin DD. B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin alpha-dependent fashion. J Exp Med. 1998; 187(7):1009-1018. (Clone-specific: Immunohistochemistry). 4. Heyman B, Wiersma EJ, Kinoshita T. In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody. J Exp Med. 1990; 172(2):665-668. (Biology). 5. Hu H, Martin BK, Weis JJ, Weis JH. Expression of the murine CD21 gene is regulated by promoter and intronic sequences. J Immunol. 1997; 158(10):4758-4768. (Biology). 6. Kinoshita T, Takeda J, Hong K, Kozono H, Sakai H, Inoue K. Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative. J Immunol. 1988; 140(9):3066-3072. (Immunogen). 7. Kinoshita T, Thyphronitis G, Tsokos GC, et al. Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor. Int Immunol. 1990; 2(7):651-659. (Biology). 8. Kurtz CB, O'Toole E, Christensen SM, Weis JH. The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1. J Immunol. 1990; 144(9):3581-3591. (Biology). 9. Martin BK, Weis JH. Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products. Eur J Immunol. 1993; 23(11):3037-3042. (Biology). 10. Molina H, Holers VM, Li B, et al. Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2. Proc Natl Acad Sci U S A. 1996; 93(8):3357-3361. (Biology). 11. Wiersma EJ, Kinoshita T, Heyman B. Inhibition of immunological memory and T-independent humoral responses by monoclonal antibodies specific for murine complement receptors. Eur J Immunol. 1991; 21(10):2501-2506. (Biology). 12. Yoshida K, van den Berg TK, Dijkstra CD. Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping. Immunology. 1993; 80(1):34-39. (Biology).

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