CD1d

Rat LEW, also known as Lewis IgG2b, κ

Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma and L929 cells

The 1B1 monoclonal antibody specifically binds to mouse CD1d, a 48-kDa glycoprotein with structural homology to major histocompatibility complex (MHC) class I molecules. The structure, expression, and functions of CD1 antigens are complex and have been reviewed. MAb 1B1 detects CD1d at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus. It appears to recognize CD1d only in association with β2m. CD1d has been reported to be expressed by gastrointestinal tract epithelium and in the cytoplasm of hepatocytes via immunohistochemical staining of frozen sections with mAb 3C11 (Cat. No. 559871, for the purified antibody), suggesting a possible role for CD1d in mucosal immunity. However, CD1d expression was not detectable via flow cytometry on intestinal epithelial cells in studies using the anti-CD1d mAbs 3C11, 1B1, and 9C7. The 1B1 antibody competes with mAb 3C11 in binding to mouse splenocytes.   This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

1B1 The 1B1 monoclonal antibody specifically binds to mouse CD1d, a 48-kDa glycoprotein with structural homology to major histocompatibility complex (MHC) class I molecules. The structure, expression, and functions of CD1 antigens are complex and have been reviewed. MAb 1B1 detects CD1d at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus. It appears to recognize CD1d only in association with β2m. CD1d has been reported to be expressed by gastrointestinal tract epithelium and in the cytoplasm of hepatocytes via immunohistochemical staining of frozen sections with mAb 3C11 (Cat. No. 559871, for the purified antibody), suggesting a possible role for CD1d in mucosal immunity. However, CD1d expression was not detectable via flow cytometry on intestinal epithelial cells in studies using the anti-CD1d mAbs 3C11, 1B1, and 9C7. The 1B1 antibody competes with mAb 3C11 in binding to mouse splenocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Rat LEW, also known as Lewis IgG2b, κ

克隆 1B1 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)

Mouse (QC Testing)

CD1d

Cd1d1; Cd1.1; Cd1a; Cd1d; Ly-38

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(9) 1. Amano M, Baumgarth N, Dick MD, et al. CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms. J Immunol. 1998; 161(4):1710-1717. (Clone-specific: Blocking, Inhibition). 2. Bleicher PA, Balk SP, Hagen SJ, Blumberg RS, Flotte TJ, Terhorst C. Expression of murine CD1 on gastrointestinal epithelium. Science. 1990; 250(4981):679-682. (Biology). 3. Brossay L, Jullien D, Cardell S, et al. Mouse CD1 is mainly expressed on hemopoietic-derived cells. J Immunol. 1997; 159(3):1216-1224. (Immunogen: Blocking, Immunoprecipitation). 4. Kawano T, Cui J, Koezuka Y, et al. CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides. Science. 1997; 278(5343):1626-1629. (Clone-specific: Blocking). 5. Porcelli SA, Modlin RL. The CD1 system: antigen-presenting molecules for T cell recognition of lipids and glycolipids. Annu Rev Immunol. 1999; 17:297-329. (Biology). 6. Roark JH, Park SH, Jayawardena J, Kavita U, Shannon M, Bendelac A. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J Immunol. 1998; 160(7):3121-3127. (Clone-specific: Blocking, Inhibition). 7. Schofield L, McConville MJ, Hansen D, et al. CD1d-restricted immunoglobulin G formation to GPI-anchored antigens mediated by NKT cells. Science. 1999; 283(5399):225-229. (Clone-specific: Blocking). 8. Sydora BC, Brossay L, Hagenbaugh A, Kronenberg M, Cheroutre H. TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes. J Immunol. 1996; 156(11):4209-4216. (Biology). 9. Szalay G, Ladel CH, Blum C, Brossay L, Kronenberg M, Kaufmann SH. Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice. J Immunol. 1999; 162(12):6955-6958. (Clone-specific: Inhibition).

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