产品信息
荧光素标记
FITC
抗原名称
IgM
宿主
Mouse IgG1, κ
免疫原
Pooled Rat Ig
简单描述
The G53-238 antibody reacts specifically with rat IgM monomers and pentamers. It does not react with other Ig isotypes. G53-238 antibody has not been shown to stimulate B-cell proliferation.
商品描述
G53-238
The G53-238 antibody reacts specifically with rat IgM monomers and pentamers. It does not react with other Ig isotypes. G53-238 antibody has not been shown to stimulate B-cell proliferation.
同种型
Mouse IgG1, κ
克隆号
克隆 G53-238 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Rat (QC Testing)
目标/特异性
IgM
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
参考图片
Detection of intracellular rat IgM in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described below using FITC-conjugated G53-238 mAb (filled histogram) or the matched istotype control, FITC-conjugated MOPC-21 mAb (open histogram, Cat. No. 554679). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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