BD Pharmingen- PerCP Hamster IgG1- - Isotype Control_BD Pharmingen
产品信息
荧光素标记
PerCP
免疫原
TNP-keyhole limpet hemocyanin
简单描述
The A19-3 monoclonal antibody specifically binds to the hapten, trinitrophenol (TNP). TNP is not expressed on human, mouse, or rat cells. The immunoglobulin from clone A19-3 was selected as an isotype control following screening for low background staining on a variety of mouse and human tissues.
商品描述
A19-3
The A19-3 monoclonal antibody specifically binds to the hapten, trinitrophenol (TNP). TNP is not expressed on human, mouse, or rat cells. The immunoglobulin from clone A19-3 was selected as an isotype control following screening for low background staining on a variety of mouse and human tissues.
同种型
Armenian Hamster IgG1, κ
克隆号
克隆 A19-3 (RUO)
浓度
0.2 mg/ml
产品详情
PerCP
PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP
Blue 488 nm
481 nm
675 nm
应用
实验应用
Flow cytometry, Isotype control (Routinely Tested)
目标/特异性
Isotype Control Arm Hamster IgG1k
背景
别名
Anti-Trinitrophenol (TNP); TNP (Isotype Control Arm)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Afar B, Merrill J, Clark EA. Detection of lymphocyte subsets using three-color/single-laser flow cytometry and the fluorescent dye peridinin chlorophyll-alpha protein. J Clin Immunol. 1991; 11(5):254-261. (Methodology).
2. Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J. Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets. Cytometry. 1996; 23(3):205-217. (Methodology).
3. Shapiro HM. Practical Flow Cytometry, 3rd Edition. New York: Wiley-Liss, Inc; 1995:280-281.
4. Waggoner AS, Ernst LA, Chen CH, Rechtenwald DJ. PE-CY5. A new fluorescent antibody label for three-color flow cytometry with a single laser. Ann N Y Acad Sci. 1993; 677:185-193. (Methodology).
参考图片
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