BD Pharmingen- PE Rat IgG2b- - Isotype Control_BD Pharmingen
产品信息
荧光素标记
PE
免疫原
TNP-Keyhole Limpet Hemocyanin
简单描述
The A95-1 antibody has unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten that is not expressed on human, mouse, rat, or non-human primate cells. The A95-1 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
商品描述
A95-1
The A95-1 antibody has unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten that is not expressed on human, mouse, rat, or non-human primate cells. The A95-1 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
克隆号
克隆 A95-1 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
目标/特异性
Rat IgG2b κ Isotype Ctrl
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology).
参考图片
Expression of IL-10 by MiCK-2 Cytokine Positive Control Cells. MiCK-2 cells (Cat. No. 554653) were permeabilized, and subsequently stained with 0.12 µg of PE-conjugated rat anti-mouse IL-10 antibody (PE-JES5-16E3, Cat. No. 554467; right panel) or 0.12 µg of PE-A95-1 rat IgG2b isotype control immunoglobulin (Cat. No. 556925; left panel) by using the BD Pharmingen staining protocol. The quadrant markers for the bivariate dot plots were set based on autofluorescence control.
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