产品信息
抗原名称
ERK1
宿主
Mouse IgG2b
免疫原
C-Terminal Region of ERK1
简单描述
A serine/threonine specific protein kinase, called MAP kinase or more recently ERK1 (extracellular signal regulated kinase) is activated in cells following stimulation with EGF (epidermal growth factor), the PDGF (platelet-derived factor) or insulin. ERK1 has been assayed
in vitro
for phosphotransferase activity using the microtubule-associated protein (MAP) or myelin basic protein. The mechanism by which MAP kinase becomes activated in growth factor treated cells is thought to involve tyrosine and threonine phosphorylation of the kinase itself. The EGF, PDGF and insulin receptors are known to be tyrosine specific protein kinases and studies have shown that MAP kinase is identical to the p42 protein that earlier studies identified as one of the major tyrosine phosphorylated proteins in transformed or growth factor treated cells. While several isoforms of the p42 protein are known, they are thought to be structurally similar. The G262-118 antibody recognizes human and mouse ERK1. It does not cross-react with ERK2. A synthetic peptide from the C-terminal region of ERK1 was used as immunogen.
商品描述
G262-118
A serine/threonine specific protein kinase, called MAP kinase or more recently ERK1 (extracellular signal regulated kinase) is activated in cells following stimulation with EGF (epidermal growth factor), the PDGF (platelet-derived factor) or insulin. ERK1 has been assayed
in vitro
for phosphotransferase activity using the microtubule-associated protein (MAP) or myelin basic protein. The mechanism by which MAP kinase becomes activated in growth factor treated cells is thought to involve tyrosine and threonine phosphorylation of the kinase itself. The EGF, PDGF and insulin receptors are known to be tyrosine specific protein kinases and studies have shown that MAP kinase is identical to the p42 protein that earlier studies identified as one of the major tyrosine phosphorylated proteins in transformed or growth factor treated cells. While several isoforms of the p42 protein are known, they are thought to be structurally similar. The G262-118 antibody recognizes human and mouse ERK1. It does not cross-react with ERK2. A synthetic peptide from the C-terminal region of ERK1 was used as immunogen.
同种型
Mouse IgG2b
克隆号
克隆 G262-118 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Immunoprecipitation (Not Recommended)
反应种属
Mouse (QC Testing), Human (Tested in Development)
目标/特异性
ERK1
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Campos-Gonzalez R, Glenney JR Jr. Temperature-dependent tyrosine phosphorylation of microtubule-associated protein kinase in epidermal growth factor-stimulated human fibroblasts. Cell Regul. 1993; 2(8):663-673. (Biology).
2. Rossomando AJ, Payne DM, Weber MJ, Sturgill TW. Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase. Proc Natl Acad Sci U S A. 1989; 86(18):6940-6943. (Biology).
参考图片
Western blot analysis of ERK1. RSVtransformed mouse 3T3 fibroblasts were probed with anti-ERK1 (clone G262-118) at concentrations of 0.2 (lane 1), 0.04 (lane 2), and 0.008 µg/ml (lane 3). ERK1 is detected at ~44 kDa.
Western blot analysis of ERK1. RSVtransformed mouse 3T3 fibroblasts were probed with anti-ERK1 (clone G262-118) at concentrations of 0.2 (lane 1), 0.04 (lane 2), and 0.008 µg/ml (lane 3). ERK1 is detected at ~44 kDa.
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