Cyclin D1

Mouse IgG1

Recombinant full-length human cyclin D1

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. Several classes of cyclins (A-E) have been described. Cyclins have been placed into functional groups as follows: Group 1 (cyclins A, B, D1, D2, D3, E and F) functions primarily in cell cycle reguation; Group 2 (cyclins C and H) also plays a role in transcriptional regulation; Group 3 (cyclins G1, G2 and I) may play a role distinct from other cyclins. Specific substrates for cyclin-cdk complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T Ag), tumor suppressor genes (Rb and p53), and others. The D-type cyclins are involved in regulating the passage of mammalian cells through G1. The reduced molecular weights of cyclin D1 is ~36 kD. G124-326 recognizes human cyclin D1 and cross-reacts with the mouse homolog (Cyl1) of human cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human cyclin D1 was used as immunogen. The antibody was originally evaluated by ELISA, western blot analysis and immunohistochemistry of frozen and paraffin-embedded tissue sections.

G124-326 Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. Several classes of cyclins (A-E) have been described. Cyclins have been placed into functional groups as follows: Group 1 (cyclins A, B, D1, D2, D3, E and F) functions primarily in cell cycle reguation; Group 2 (cyclins C and H) also plays a role in transcriptional regulation; Group 3 (cyclins G1, G2 and I) may play a role distinct from other cyclins. Specific substrates for cyclin-cdk complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T Ag), tumor suppressor genes (Rb and p53), and others. The D-type cyclins are involved in regulating the passage of mammalian cells through G1. The reduced molecular weights of cyclin D1 is ~36 kD. G124-326 recognizes human cyclin D1 and cross-reacts with the mouse homolog (Cyl1) of human cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human cyclin D1 was used as immunogen. The antibody was originally evaluated by ELISA, western blot analysis and immunohistochemistry of frozen and paraffin-embedded tissue sections.

Mouse IgG1

克隆 G124-326 (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Flow cytometry, Immunohistochemistry, Immunoprecipitation (Tested During Development)

Human (QC Testing), Mouse (Reported)

Cyclin D1

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Bates S, Rowan S, Vousden KH. Characterisation of human cyclin G1 and G2: DNA damage inducible genes. Oncogene. 1996; 13(5):1103-1109. (Biology). 2. Gong J, Traganos F, Darzynkiewicz Z. Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase. Cell Prolif. 1995; 28(6):337-346. (Clone-specific: Flow cytometry, Western blot). 3. Meyerson M, Harlow E. Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 1994; 14(3):2077-2086. (Biology). 4. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines. Cancer Res. 1995; 55(3):505-509. (Clone-specific: Immunohistochemistry, Western blot). 5. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology).