E2F-1

Mouse IgG2a

Human E2F-1 recombinant protein aa. 1-437

The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor bound to the adenovirus E2A promoter. E2F-binding sites have now been identified in cellular promoters for genes involved in growth regulation, including c-myc, N-myc, Cdc2 and cyclin A. They have also been identified in promoters for genes whose products are required for DNA synthesis and replication, such as DNA polymerase a, thymidine kinase, thymidylate synthase, and dihydrofolate reductase. When bound to DNA, E2F has been found in complexes with key regulators of cell proliferation including the retinoblastoma protein (Rb) and the Rb related proteins, p107 and p130. Like a variety of other transcription factors, E2F binds to DNA as a heterodimer. The protein E2F-1 has E2F-like properties, it mediates E2F-dependent trans-activation and binds to underphosphorylated Rb. E2F dimerizes with DP-1, a protein which also has E2F-like properties. This dimerization leads to increased DNA binding activity and enhanced transcriptional activity. It is thought that E2F-1/DP association forms an active "E2F" transcription factor. E2F-1 migrates at a reduced molecular weight of ~60 kDa. Clone KH95/E2F (also referred to as KH95) recognizes an epitope between amino acids 342 and 386 of human E2F-1. It cross reacts with mouse and rat E2F-1. Full length, bacterially expressed, recombinant histidine-tagged human E2F-1 (amino acids 1-437) was purified and used as immunogen. Positive tissue culture supernatants were identified by immunoprecipitation of [35S]methionine-labeled, in vitro synthesized E2F-1 protein.

KH95/E2F The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor bound to the adenovirus E2A promoter. E2F-binding sites have now been identified in cellular promoters for genes involved in growth regulation, including c-myc, N-myc, Cdc2 and cyclin A. They have also been identified in promoters for genes whose products are required for DNA synthesis and replication, such as DNA polymerase a, thymidine kinase, thymidylate synthase, and dihydrofolate reductase. When bound to DNA, E2F has been found in complexes with key regulators of cell proliferation including the retinoblastoma protein (Rb) and the Rb related proteins, p107 and p130. Like a variety of other transcription factors, E2F binds to DNA as a heterodimer. The protein E2F-1 has E2F-like properties, it mediates E2F-dependent trans-activation and binds to underphosphorylated Rb. E2F dimerizes with DP-1, a protein which also has E2F-like properties. This dimerization leads to increased DNA binding activity and enhanced transcriptional activity. It is thought that E2F-1/DP association forms an active "E2F" transcription factor. E2F-1 migrates at a reduced molecular weight of ~60 kDa. Clone KH95/E2F (also referred to as KH95) recognizes an epitope between amino acids 342 and 386 of human E2F-1. It cross reacts with mouse and rat E2F-1. Full length, bacterially expressed, recombinant histidine-tagged human E2F-1 (amino acids 1-437) was purified and used as immunogen. Positive tissue culture supernatants were identified by immunoprecipitation of [35S]methionine-labeled, in vitro synthesized E2F-1 protein.

Mouse IgG2a

克隆 KH95/E2F (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Immunoprecipitation (Reported)

Human (QC Testing), Mouse, Rat (Tested in Development)

E2F-1

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Dyson N, Dembski M, Fattaey A, Ngwu C, Ewen M, Helin K. Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1. J Virol. 1993; 67(12):7641-7647. (Clone-specific: Western blot). 2. Helin K, Wu CL, Fattaey AR, et al. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Genes Dev. 1993; 7(10):1850-1861. (Clone-specific: Immunoprecipitation, Western blot). 3. Huber HE, Edwards G, Goodhart PJ, et al. Transcription factor E2F binds DNA as a heterodimer. Proc Natl Acad Sci U S A. 1993; 90(8):3525-3529. (Biology). 4. Nevins JR. E2F: a link between the Rb tumor suppressor protein and viral oncoproteins. Science. 1992; 258(5081):424-429. (Biology). 5. Saito M, Helin K, Valentine MB, et al. Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics. 1995; 25(1):130-138. (Clone-specific: Immunoprecipitation, Western blot).