PU.1

Mouse IgG2a

PU.1 Fusion Protein

PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages.  It was initially identified as the oncogene Spi -1 which was found to block erythroblast differentiation.  PU.1 functions by binding transcriptional control elements such as µB in the immunoglobulin heavy chain enhancer and π  adjacent to µE2.  It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin κ 3'  enhancer.  PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor.  PU.1 runs as a 40-42 kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor.  PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.

G148-74 PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages.  It was initially identified as the oncogene Spi -1 which was found to block erythroblast differentiation.  PU.1 functions by binding transcriptional control elements such as µB in the immunoglobulin heavy chain enhancer and π  adjacent to µE2.  It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin κ 3'  enhancer.  PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor.  PU.1 runs as a 40-42 kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor.  PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.

Mouse IgG2a

克隆 G148-74 (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Tested During Development)

Human (QC Testing)

PU.1

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(6) 1. Klemsz MJ, McKercher SR, Celada A, Van Beveren C, Maki RA. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell. 1990; 61(1):113-124. (Biology). 2. Moreau-Gachelin F, Ray D, Mattei MG, Tambourin P, Tavitian A. The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias. Oncogene. 1989; 4(12):1449-1456. (Biology). 3. Pongubala JM, Nagulapalli S, Klemsz MJ, McKercher SR, Maki RA, Atchison ML.. PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity. Mol Cell Biol. 1992; 12(1):368-378. (Biology). 4. Rivera RR, Stuiver MH, Steenbergen R, Murre C. Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression. Mol Cell Biol. 1993; 13(11):7163-7169. (Biology). 5. Shin MK, Koshland ME. Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element. Genes Dev. 1993; 7(10):2006-2015. (Biology). 6. Zhang DE, Hetherington CJ, Chen HM, Tenen DG. The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor. Mol Cell Biol. 1994; 14(1):373-381. (Biology).