Disialoganglioside GD2

Mouse IgG3, κ

FM9 human melamona cell line

Gangliosides are sialic-acid bearing glycolipids expressed on the surface of all mammalian cells, and are likely involved in mediating cell-substratum interactions. They are important target antigens for antibody-mediated cytolysis of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides GD2 and/or GD3 which are present in substratum-attached material, and may play a significant role in the melanoma metastatic phenotype. Ganglioside GD3 is a major surface marker on most human melanoma cells. MB3.6 has been used to localize GD3 in the plasma membrane and in focal adhesion plaques of human melanoma cells. Clone MB3.6 has also been shown to lyse GD3 positive human melanoma cells by both antibody-dependent and complement-mediated cytotoxicity, as well as inhibit the growth of human melanoma cells in athymic nude mice. Clone MB3.6 specifically reacts with human GD3 ganglioside. It does not cross-react with a variety of other gangliosides purified from melanoma or neuroblastoma cells. The FM9 human melanoma cell line was used as the immunogen.

MB3.6 Gangliosides are sialic-acid bearing glycolipids expressed on the surface of all mammalian cells, and are likely involved in mediating cell-substratum interactions. They are important target antigens for antibody-mediated cytolysis of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides GD2 and/or GD3 which are present in substratum-attached material, and may play a significant role in the melanoma metastatic phenotype. Ganglioside GD3 is a major surface marker on most human melanoma cells. MB3.6 has been used to localize GD3 in the plasma membrane and in focal adhesion plaques of human melanoma cells. Clone MB3.6 has also been shown to lyse GD3 positive human melanoma cells by both antibody-dependent and complement-mediated cytotoxicity, as well as inhibit the growth of human melanoma cells in athymic nude mice. Clone MB3.6 specifically reacts with human GD3 ganglioside. It does not cross-react with a variety of other gangliosides purified from melanoma or neuroblastoma cells. The FM9 human melanoma cell line was used as the immunogen.

Mouse IgG3, κ

克隆 MB3.6 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), Cytotoxicity, Immunofluorescence, Immunohistochemistry-frozen (Reported)

Human (QC Testing)

Disialoganglioside GD2

Ganglioside GD3

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

研发参考(6) 1. Cheresh DA, Harper JR, Schulz G, Reisfeld RA. Localization of the gangliosides GD2 and GD3 in adhesion plaques and on the surface of human melanoma cells. Proc Natl Acad Sci U S A. 1984; 81(18):5767-5771. (Immunogen: Cytotoxicity, Immunofluorescence). 2. Cheresh DA, Honsik CJ, Staffileno LK, Jung G, Reisfeld RA. Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis. Proc Natl Acad Sci U S A. 1985; 82(15):5155-5159. (Clone-specific: Flow cytometry). 3. Cheresh DA, Klier FG. Disialoganglioside GD2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin. J Cell Biol. 1986; 102(5):1887-1897. (Biology). 4. Cheresh DA, Pierschbacher MD, Herzig MA, Mujoo K. Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins. J Cell Biol. 1986; 102(3):688-696. (Biology). 5. Hakomori S. Tumor-associated carbohydrate antigens. Annu Rev Immunol. 1984; 2:103-126. (Biology). 6. Mujoo K, Cheresh DA, Yang HM, Reisfeld RA. Disialoganglioside GD2 on human neuroblastoma cells: target antigen for monoclonal antibody-mediated cytolysis and suppression of tumor growth. Cancer Res. 1987; 47(4):1098-1104. (Biology).