Fas (APO-1, CD95) is an ~42 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a 40 kDa TNF family member membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. Recombinant soluble human Fas was expressed in the Baculovirus Expression Vector System and purified from insect cell lysate using an anti-Fas antibody. This form of Fas lacks the transmembrane domain of the Fas receptor, and has been referred to as FasΔTM. It has a 63-base pair deletion starting at nucleotide sequence (700) GA TCC AGA and ending at nucleotide sequence GTT TGG G (762). The purity and the integrity of the protein were verified by SDS-PAGE, and ELISA.  FasΔTM has a reduced molecular weight of ~40 kDa.

(RUO)

Western blot (Routinely Tested), ELISA Standard (Tested During Development), Functional assay (Not Recommended)

sFAS

Aqueous buffered solution containing BSA and glycerol.

Development References(3) 1. Cheng J, Zhou T, Liu C, et al. Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule. Science. 1994; 263(5154):1759-1762. (Biology). 2. Takahashi T, Tanaka M, Brannan CI, et al. Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand. Cell. 1994; 76(6):969-976. (Biology). 3. Tanaka M, Suda T, Takahashi T, Nagata S. Expression of the functional soluble form of human Fas ligand in activated lymphocytes. EMBO J. 1995; 14(6):1129-1135. (Biology).