BD Pharmingen- Biotin Rat Anti-Mouse IL-5_BD Pharmingen
产品信息
抗原名称
IL-5
宿主
Rat IgG2a
免疫原
Semi-purified T Cell Clone Supernatant
简单描述
The TRFK4 antibody reacts with mouse interleukin-5 (IL-5). The immunogen used to generate the TRFK4 hybridoma was a semi-purified T cell clone supernatant. This is a neutralizing antibody.
商品描述
TRFK4
The TRFK4 antibody reacts with mouse interleukin-5 (IL-5). The immunogen used to generate the TRFK4 hybridoma was a semi-purified T cell clone supernatant. This is a neutralizing antibody.
同种型
Rat IgG2a
克隆号
克隆 TRFK4 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
ELISA Detection (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IL-5
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA).
3. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Clone-specific: ELISA).
4. Schumacher JH, O'Garra A, Shrader B, et al. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent. J Immunol. 1988; 141(5):1576-1581. (Clone-specific: ELISA).
参考图片
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