BD Pharmingen- Purified Rat Anti-Mouse GM-CSF_BD Pharmingen
产品信息
抗原名称
GM-CSF
宿主
Rat IgG2a
免疫原
Recombinant Mouse GM-CSF
简单描述
The MP1-22E9 monoclonal antibody specifically binds to mouse Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF). The immunogen used to generate the MP1-22E9 hybridoma was yeast-expressed recombinant mouse GM-CSF. This is a neutralizing antibody.
商品描述
MP1-22E9
The MP1-22E9 monoclonal antibody specifically binds to mouse Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF). The immunogen used to generate the MP1-22E9 hybridoma was yeast-expressed recombinant mouse GM-CSF. This is a neutralizing antibody.
同种型
Rat IgG2a
克隆号
克隆 MP1-22E9 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Neutralization (Tested During Development), Western blot (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
GM-CSF
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization).
3. Nozaki S, Abrams JS, Pearce MK, Sauder DN. Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes. J Invest Dermatol. 1991 July; 97(1):10-14. (Clone-specific: ELISA, Neutralization).
4. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA).
5. Suda T, O'Garra A, MacNeil I, Fischer M, Bond MW, Zlotnik A. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas. Cell Immunol. 1990; 129(1):228-240. (Clone-specific: Neutralization).
参考图片
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