BD Pharmingen- Purified NA-LE Rat Anti-Mouse TNF_BD Pharmingen
产品信息
抗原名称
TNF
宿主
Rat IgG1, κ
免疫原
Mouse TNF Recombinant Protein
简单描述
The MP6-XT3 antibody reacts with mouse TNF (TNF-α). Cross reactivity with lymphotoxin-α (LT-α ; TNF-β) has not been determined.
商品描述
MP6-XT3
The MP6-XT3 antibody reacts with mouse TNF (TNF-α). Cross reactivity with lymphotoxin-α (LT-α ; TNF-β) has not been determined.
同种型
Rat IgG1, κ
克隆号
克隆 MP6-XT3 (RUO)
浓度
1.0 mg/ml
产品详情
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
应用
实验应用
ELISA (Routinely Tested), Neutralization (Tested During Development), Western blot (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
TNF
背景
别名
Tumor Necrosis Factor
制备和贮存
存储溶液
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
保存方式
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
文献
文献
研发参考(2)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA, Western blot).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Western blot).
参考图片
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