BD Pharmingen- PE Rat Anti-Mouse CD121b_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD121b
宿主
Rat LEW, also known as Lewis IgG2a, κ
免疫原
Mouse IL-1RII Protein
简单描述
The 4E2 monoclonal antibody specifically binds to CD121b which is also known as the IL-1 receptor type II (IL-1RII, IL-1R2) or IL-1 receptor beta (IL-1R-beta, IL-1Rβ). CD121b is encoded by the
Il1r2
gene and presents as a 68 kDa type I transmembrane glycoprotein. It is expressed on a variety of cell types including B cells, neutrophils, monocytes, and bone marrow progenitor cells. A soluble form of CD121b is produced by proteolysis especially after cellular activation. Unlike the IL-1 type I receptor (CD121a), CD121b has a short cytoplasmic domain and does not mediate IL-1 signaling. CD121b may function in a membrane or soluble form as a decoy receptor that can bind IL-1 and reduce its effective concentration and biological activity. The 4E2 antibody blocks IL-1 binding to the IL-1 receptor type II.
商品描述
4E2
The 4E2 monoclonal antibody specifically binds to CD121b which is also known as the IL-1 receptor type II (IL-1RII, IL-1R2) or IL-1 receptor beta (IL-1R-beta, IL-1Rβ). CD121b is encoded by the
Il1r2
gene and presents as a 68 kDa type I transmembrane glycoprotein. It is expressed on a variety of cell types including B cells, neutrophils, monocytes, and bone marrow progenitor cells. A soluble form of CD121b is produced by proteolysis especially after cellular activation. Unlike the IL-1 type I receptor (CD121a), CD121b has a short cytoplasmic domain and does not mediate IL-1 signaling. CD121b may function in a membrane or soluble form as a decoy receptor that can bind IL-1 and reduce its effective concentration and biological activity. The 4E2 antibody blocks IL-1 binding to the IL-1 receptor type II.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 4E2 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD121b (IL-1 Receptor 2)
背景
别名
Il1r2; IL-1RII; IL-1R2; Il1rb; IL-1Rβ; IL-1R-beta; IL-1 receptor beta chain
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(7)
1. Colotta F, Re F, Muzio M, et al. Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4. Science. 1993; 261(5120):472-475. (Biology).
2. Cumberbatch M, Dearman RJ, Kimber I. Characteristics and regulation of the expression on interleukin 1 receptors by murine Langerhans cells and keratinocytes. Arch Dermatol Res. 1998; 290(12):688-695. (Clone-specific: Flow cytometry).
3. Hestdal K, Ruscetti FW, Chizzonite R, et al. Interleukin-1 (IL-1) directly and indirectly promotes hematopoietic cell growth through type I IL-1 receptor. Blood. 1994; 84(1):125-132. (Clone-specific).
4. Hunter CA, Chizzonite R, Remington JS. IL-1 beta is required for IL-12 to induce production of IFN-gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. J Immunol. 1995; 155(9):4347-4354. (Clone-specific: Neutralization).
5. McMahan CJ, Slack JL, Mosley B, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J. 1991; 10(10):2821-2832. (Biology).
6. Oldenburg HS, Pruitt JH, Lazarus DD, et al. Interleukin 1 binding to its type I, but not type II receptor, modulates the in vivo acute phase response. Cytokine. 1995; 7(6):510-516. (Clone-specific).
7. Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
参考图片
Flow cytometric analysis of mouse CD121b. Balb/c bone marrow was stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, shaded histogram) or PE Rat anti-Mouse CD121b (open histogram).
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