Purified NA-LE Rat Anti-Mouse IL-12 -p40-p70-C17-8_BD Pharmingen
产品信息
荧光素标记
Unconjugated
抗原名称
IL-12
宿主
Rat IgG2a
免疫原
Recombinant Mouse IL-12 p70
简单描述
The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.
商品描述
The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.
同种型
Rat IgG2a
克隆号
C17.8
浓度
1.0 mg/ml
产品详情
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
应用
实验应用
ELISA (Routinely Tested) Neutralization (Tested During Development) Immunoprecipitation, Western blot (Reported)
反应种属
Mouse (QC Testing)
制备和贮存
存储溶液
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
保存方式
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
文献
文献
研发参考(4)
1. D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Biology).
2. Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology).
3. Neurath MF, Fuss I, Kelsall BL, Stuber E, Strober W. Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med. 1995; 182(5):1281-1290. (Biology).
4. Wysocka M, Kubin M, Vieira LQ, et al. Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice. Eur J Immunol. 1995; 25(3):672-676. (Clone-specific: Immunoprecipitation, Western blot).
数据库链接
Entrez-Gene ID
16160
参考图片
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