BD Pharmingen- Biotin Rat Anti-Human IL-7_BD Pharmingen
产品信息
抗原名称
IL-7
宿主
Rat IgG2a
免疫原
Recombinant Human IL-7
简单描述
Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) reacts with human interleukin-7 (IL-7). This antibody can be useful as a detection antibody for sandwich ELISA measuring human IL-7 protein levels. Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) can be paired with Purified Rat Anti-Human IL-7 antibody (clone BVD10-40F6; Cat. No. 554493) as the capture antibody along with recombinant human IL-7 (Cat. No. 554608) as the standard. Investigators are advised to titrate to determine optimal usage concentrations (ELISA), with a suggested range of 2 ug/mL to 0.5 ug/mL for Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10). To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate. This ELISA pairing has been reported to demonstrate limited to no cross-reactivity with the following cytokines: Mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF or Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL- 9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF.
商品描述
BVD10-11C10
Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) reacts with human interleukin-7 (IL-7). This antibody can be useful as a detection antibody for sandwich ELISA measuring human IL-7 protein levels. Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) can be paired with Purified Rat Anti-Human IL-7 antibody (clone BVD10-40F6; Cat. No. 554493) as the capture antibody along with recombinant human IL-7 (Cat. No. 554608) as the standard. Investigators are advised to titrate to determine optimal usage concentrations (ELISA), with a suggested range of 2 ug/mL to 0.5 ug/mL for Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10). To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate. This ELISA pairing has been reported to demonstrate limited to no cross-reactivity with the following cytokines: Mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF or Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL- 9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF.
同种型
Rat IgG2a
克隆号
克隆 BVD10-11C10 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
ELISA Detection (Routinely Tested), Western blot (Reported)
反应种属
Human (QC Testing)
目标/特异性
IL-7
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology: ELISA).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology: ELISA).
参考图片
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