Purified Rat Anti-Human GM-CSF-BVD2-23B6_BD Pharmingen
产品信息
荧光素标记
Unconjugated
抗原名称
GM-CSF
宿主
Rat IgG2a
免疫原
E. coli-expressed recombinant human GM-CSF
简单描述
The BVD2-23B6 antibody reacts with human granulocyte/macrophage - colony stimulating factor (GM-CSF). The immunogen used to generate the BVD2-23B6 hybridoma was
E. coli
-expressed recombinant human GM-CSF. This is a neutralizing antibody.
This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
The BVD2-23B6 antibody reacts with human granulocyte/macrophage - colony stimulating factor (GM-CSF). The immunogen used to generate the BVD2-23B6 hybridoma was
E. coli
-expressed recombinant human GM-CSF. This is a neutralizing antibody.
This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG2a
克隆号
BVD2-23B6
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture (Routinely Tested) Neutralization, Immunoprecipitation/Western blot (Tested During Development)
反应种属
Human (QC Testing)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Immunoprecipitation, Neutralization).
2. Abrams JS, Silver JE, Van Dyke RE, Gleich GI. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GMCSF. In: Gleich GJ and Kay AB, ed. Eosinophils in Allergy and Inflammation. New York: Dekker; 1994:133-157.
3. Bacchetta R, de Waal Malefijt R, Yssel H. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990; 144(3):902-908. (Clone-specific: ELISA, Neutralization).
4. Kita H, Ohnishi T, Okubo Y, Weiler D, Abrams JS, Gleich GJ. Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils. J Exp Med. 1991; 174(3):745-748. (Clone-specific: ELISA, Neutralization).
参考图片
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