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BD Pharmingen- PE Mouse Anti-Human IL-12 -p40-p70_BD Pharmingen

艾美捷科技 • 2025年08月22日 11:26:42 • 抗体 • 阅读 13 • 标签
产品信息
荧光素标记
PE
抗原名称
IL-12
宿主
Mouse IgG1
免疫原
CHO-expressed recombinant human IL-12 p70 heterodimer
简单描述
The C11.5 monoclonal antibody specifically binds to the human IL-12 p40 monomer and p70 heterodimer, but does not bind to the IL-12 p35 monomer. The immunogen used to generate the C11.5 hybridoma was the CHO-expressed recombinant human IL-12 p70 heterodimer.  p40 has also been described as a subunit of IL-23 and thus it is possible that the C11.5 antibody crossreacts with IL-23.
商品描述
C11.5 The C11.5 monoclonal antibody specifically binds to the human IL-12 p40 monomer and p70 heterodimer, but does not bind to the IL-12 p35 monomer. The immunogen used to generate the C11.5 hybridoma was the CHO-expressed recombinant human IL-12 p70 heterodimer.  p40 has also been described as a subunit of IL-23 and thus it is possible that the C11.5 antibody crossreacts with IL-23.
同种型
Mouse IgG1
克隆号
克隆 C11.5 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
IL-12
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5) 1. D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G. Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells. J Exp Med. 1993; 178(3):1041-1048. (Clone-specific). 2. D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Clone-specific). 3. Gately MK, Chizzonite R, Presky DH. Measurement of Human and Mouse Interleukin-12. In: Cooligan J, Kruisbeek A, Margulies D, Shevach E, Storber W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1995:6-16. 4. Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Biology). 5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology).

参考图片

Expression of IL-12 p40/p70 by activated CD14+ human PBMCs. Ficoll-separated human PBMCs were primed for 2 hours with recombinant human IFN-γ (10 ng/ml final concentration; then activated with IFN-γ (10 ng/ml final concentration) and LPS (100 ng/ml final concentration; Sigma) in the presence of GolgiStop™ (2 µM final concentration) for an additional 22 hours. Cells were harvested, stained with FITC-mouse anti human CD14 antibody fixed, permeabilized, and then stained with 0.125 µg of PE-C11.5 antibody following our staining protocol (far left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of PE-C11.5 antibody was blocked by preincubation of the conjugated antibody with excess recombinant human IL-12 p70 (0.25 µg) and recombinant human IL-12 p40, but not by recombinant human IL-12 p35 protein. Preincubation of the fixed/permeabilized cells with an excess of the unlabelled C11.5 antibody prior to staining with the PE-C11.5 antibody also blocked staining (data not shown). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabelled antibody blocking specificity controls.

Expression of IL-12 p40/p70 by activated CD14+ human PBMCs. Ficoll-separated human PBMCs were primed for 2 hours with recombinant human IFN-γ (10 ng/ml final concentration; then activated with IFN-γ (10 ng/ml final concentration) and LPS (100 ng/ml final concentration; Sigma) in the presence of GolgiStop™ (2 µM final concentration) for an additional 22 hours. Cells were harvested, stained with FITC-mouse anti human CD14 antibody fixed, permeabilized, and then stained with 0.125 µg of PE-C11.5 antibody following our staining protocol (far left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of PE-C11.5 antibody was blocked by preincubation of the conjugated antibody with excess recombinant human IL-12 p70 (0.25 µg) and recombinant human IL-12 p40, but not by recombinant human IL-12 p35 protein. Preincubation of the fixed/permeabilized cells with an excess of the unlabelled C11.5 antibody prior to staining with the PE-C11.5 antibody also blocked staining (data not shown). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabelled antibody blocking specificity controls.

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