Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30% of the molecule by weight.3 It shares 80% and 95% homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys. Formulation and Purity Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay .

(RUO)

0.1 mg/ml

ELISA (Routinely Tested), Bioassay (Tested During Development)

Mouse (QC Testing)

Epo

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(7) 1. Jacobson LO, Goldwasser E, Fried W, Plzak L. Role of the kidney in erythropoiesis. Nature. 1957; 179(4560):633-634. (Biology). 2. Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Biology). 3. Krantz SB. Erythropoietin. Blood. 1991; 77(3):419-434. (Biology). 4. McDonald JD, Lin FK, Goldwasser E. Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene. Mol Cell Biol. 1986; 6(3):842-848. (Biology). 5. Nagao, M., H. Suga, et al. Nucleotide sequence of rat erythropoietin. Biochim Biophys Acta. 1992; 1171(1):99-102. (Biology). 6. Shoemaker CB, Mitsock LD. Murine erythropoietin gene: cloning, expression, and human gene homology. Mol Cell Biol. 1986; 6(3):849-858. (Biology). 7. Wu H, Liu X, Jaenisch R, Lodish HF. Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor. Cell. 1995; 83(1):59-67. (Biology).