Interleukin-5 (IL-5) is the lymphokine responsible for the activities attributed to eosinophil differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF).  IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation.  Recombinant human IL-5 is a 28 - 31 kD protein dimer as determined by SDS-PAGE (non-reducing conditions).  Recombinant human IL-5 (Cat. No. 554606) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives.  Recombinant human IL-5 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng per µg of human IL-5, as measured in chromogenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Intracellular block/flow cytometry (Tested During Development)

Human (QC Testing)

IL-5 recombinant

Frozen aqueous buffered solution containing BSA.

Development References(3) 1. Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Methodology: Bioassay). 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). 3. Sideras P, Noma T, Honjo T. Structure and function of interleukin 4 and 5. Immunol Rev. 1988; 102:189-212. (Biology).